YRDC Polyclonal antibody

YRDC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26295-1-AP

Synonyms

DRIP3, hIRIP, IRIP, RP11 109P14.4, SUA5, YRDC

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Validation Data Gallery

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  WB analysis of HEK-293T using 26295-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26295-1-AP (YRDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 26295-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 26295-1-AP (YRDC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 26295-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26295-1-AP (YRDC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 26295-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26295-1-AP (YRDC Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 26295-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 26295-1-AP (YRDC Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293T cells, BxPC-3 cells, RAW 264.7 cells
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26295-1-AP targets YRDC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: YRDC fusion protein Ag23633
• Full Name: yrdC domain containing (E. coli)
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC080186
• Gene Symbol: YRDC
• Gene ID (NCBI): 79693
• RRID: AB_2880468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.