Tested Applications
| Positive WB detected in | LNCaP cells, MDA-MB-231 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67911-1-Ig targets YTHDC1 in WB, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6289 Product name: Recombinant human YTHDC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 360-709 aa of BC053863 Sequence: STLPVNEKKLNLAFRSARSVILIFSVRESGKFQGFARLSSESHHGGSPIHWVLPAGMSAKMLGGVFKIDWICRRELPFTKSAHLTNPWNEHKPVKIGRDGQEIELECGTQLCLLFPPDESIDLYQVIHKMRHKRRMHSQPRSRGRPSRREPVRDVGRRRPEDYDIHNSRKKPRIDYPPEFHQRPGYLKDPRYQEVDRRFSGVRRDVFLNGSYNDYVREFHNMGPPPPWQGMPPYPGMEQPPHHPYYQHHAPPPQAHPPYSGHHPVPHEARYRDKRVHDYDMRVDDFLRRTQAVVSGRRSRPRERDRERERDRPRDNRRDRERDRGRDRERERERLCDRDRDRGERGRYRR Predict reactive species |
| Full Name | YTH domain containing 1 |
| Calculated Molecular Weight | 85 kDa |
| Observed Molecular Weight | ~100 kDa |
| GenBank Accession Number | BC053863 |
| Gene Symbol | YTHDC1 |
| Gene ID (NCBI) | 91746 |
| RRID | AB_2918666 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q96MU7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
YTHDC1, containing 1 YTH domain, is one of the m6A-binding proteins that can recognize and bind to the m6A methylation site and plays a specific role in gene expression. YTHDC1 is constitutively enriched in the nucleus. It regulates mRNA splicing by bridging the interactions between the trans- and cis-regulatory elements to bind targeted mRNAs. YTHDC1 mediates the export of m6A modified mRNA from the nucleus to the cytoplasm by interacting with SRSF3, which is an essential factor driving the tumorigenic process in various types of cancers including breast cancer, colon cancer, ovarian cancer, osteosarcoma, and glioblastoma (32368386).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for YTHDC1 antibody 67911-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The YTHDC1 Monoclonal antibody (1F5G11), catalog number 67911-1-Ig, has 2 citations. These appear in Advanced Science (2026) and Molecular Carcinogenesis (2024). The cited research spans cardiovascular biology (Ythdc1-mediated Golgi dysfunction in aortic aneurysm) and oncology (m6A modification of VEGFA mRNA in hepatocellular carcinoma angiogenesis). Applications used include Western blot and RNA immunoprecipitation, with reactivity in human and mouse.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Ythdc1-p300-Klf5 Complex-Mediated Golgi Dysfunction Promotes Aortic Aneurysm. | ||
Mol Carcinog m6A modification of VEGFA mRNA by RBM15/YTHDF2/IGF2BP3 contributes to angiogenesis of hepatocellular carcinoma |
Reviews
What customers say
Both reviewers rated this antibody 5 stars for Western Blot. They reported good quality with clear, specific bands. One user detected YTHDC1 at approximately 100 kDa in 293T cells using a 1:4000 dilution. The other confirmed a clear band in HEK293T cells. Overall, customers found the antibody reliable and effective for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zhongwen (Verified Customer) (09-25-2023) | Good quality with clear band.
|
Xingyuan (Verified Customer) (06-30-2023) | Quality was good. Did show clear band for YTHDC1 at around 100kDa for the western blot.
 |
