YTHDF1 Polyclonal antibody

YTHDF1 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 26787-1-AP

Synonyms

C20orf21, DACA 1, YTH domain family protein 1, YTH domain family, member 1, YTHDF1



Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26787-1-AP is YTHDF1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YTHDF1 fusion protein Ag25347
Full Name YTH domain family, member 1
Calculated molecular weight 559 aa, 61 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC050284
Gene symbol YTHDF1
Gene ID (NCBI) 54915
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YTHDF1, also named as YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoform and calculated molecular weight of isoforms are 61 kDa and 21 kDa.

Protocols

Product Specific Protocols
WB protocol for YTHDF1 antibody 26787-1-APDownload protocol
IP protocol for YTHDF1 antibody 26787-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols