YTHDF1 Polyclonal antibody

YTHDF1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26787-1-AP

Synonyms

C20orf21, DACA 1, YTH domain family protein 1, YTH domain family, member 1, YTHDF1



Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26787-1-AP is YTHDF1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YTHDF1 fusion protein Ag25347
Full Name YTH domain family, member 1
Calculated molecular weight 559 aa, 61 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC050284
Gene symbol YTHDF1
Gene ID (NCBI) 54915
RRIDAB_2880635
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YTHDF1, also named YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at the internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing, and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoforms and the calculated molecular weight of isoforms are 61 kDa and 21 kDa.

Protocols

Product Specific Protocols
WB protocol for YTHDF1 antibody 26787-1-APDownload protocol
IP protocol for YTHDF1 antibody 26787-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Cell Mol Neurobiol

Wnt3a/YTHDF1 Regulated Oxaliplatin-Induced Neuropathic Pain Via TNF-α/IL-18 Expression in the Spinal Cord

Authors - Xiaohui Bai
humanWB

Cancer Med

BIRC5 facilitates cisplatin-chemoresistance in a m6 A-dependent manner in ovarian cancer

Authors - Yadan Fan