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  • KD/KO Validated

YWHAZ Polyclonal antibody

YWHAZ Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP

Conjugate

Unconjugated

Cat no : 14881-1-AP

Synonyms

14 3 3 protein zeta/delta, 14-3-3, KCIP 1, YWHAZ



Tested Applications

Positive WB detected inSH-SY5Y cells, Jurkat cells, Raji cells, mouse brain, rat brain

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Sample-dependent, check data in validation data gallery

Product Information

14881-1-AP targets YWHAZ in WB, IHC, IF, CoIP applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YWHAZ fusion protein Ag6654
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
Calculated molecular weight 28 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC003623
Gene symbol YWHAZ
Gene ID (NCBI) 7534
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YWHAZ (also known as 14-3-3 zeta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against the full-length YWHAZ.

Protocols

Product Specific Protocols
WB protocol for YWHAZ antibody 14881-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Oncogene

Lysine-specific demethylase 1 promotes the stemness and chemoresistance of Lgr5(+) liver cancer initiating cells by suppressing negative regulators of β-catenin signaling.

Authors - Z-J Lei
  • KD Validated
humanWB

Oncotarget

c-Myc suppresses miR-451⊣YWTAZ/AKT axis via recruiting HDAC3 in acute myeloid leukemia.

Authors - Rui Su
humanWB, IHC

Cell Death Discov

YWHAZ interacts with DAAM1 to promote cell migration in breast cancer.

Authors - Jie Mei
humanWB,IF,CoIP

Biomed Environ Sci

YWHAZ Binds to TRIM21 but Is Not Involved in TRIM21-stimulated Osteosarcoma Cell Proliferation.

Authors - Qing Zhong Zeng
humanWB

Dis Model Mech

Integrated lipidomic and transcriptomic analyses identify altered nerve triglycerides in mouse models of prediabetes and type 2 diabetes.

Authors - Phillipe D O'Brien
humanWB

Biochem Pharmacol

1,3-Dicaffeoylquinic acid targeting 14-3-3 tau suppresses human breast cancer cell proliferation and metastasis through IL6/JAK2/PI3K pathway.

Authors - Yuhang Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Erik (Verified Customer) (08-26-2021)

We used the antibody at 1:1000 dilution with 11ug input protein, and it produced one clean and strong band at the expected height for the protein

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MIAPACA2 and PANC1 cell lines
YWHAZ Antibody Western Blot validation (1:1000 dilution) in MIAPACA2 and PANC1 cell lines (Cat no:14881-1-AP)