"YY1 Antibodies" Comparison
View side-by-side comparison of YY1 antibodies from other vendors to find the one that best suits your research needs.
|Positive WB detected in||Jurkat cells, A549 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22156-1-AP is YY1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, bovine, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||YY1 fusion protein Ag17792|
|Full Name||YY1 transcription factor|
|Calculated molecular weight||414 aa, 45 kDa|
|Observed molecular weight||65-70 kDa|
|GenBank accession number||BC037308|
|Gene ID (NCBI)||7528|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. This is a rabbit polyclonal antibody raised against full length YY1 of human origin.
|Product Specific Protocols|
|WB protocol for YY1 antibody 22156-1-AP||Download protocol|
|IHC protocol for YY1 antibody 22156-1-AP||Download protocol|
|IF protocol for YY1 antibody 22156-1-AP||Download protocol|
|IP protocol for YY1 antibody 22156-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biochem Biophys Res Commun
YY1-regulated LINC00152 promotes triple negative breast cancer progression by affecting on stability of PTEN protein.
A haplotype variant of Hu sheep follicle-stimulating hormone receptor promoter region decreases transcriptional activity.
Ann Transl Med
Heat shock cognate protein 70 promotes the differentiation of C2C12 myoblast and targets Yin Yang 1.
Cell Death Dis
Long non-coding RNA Xist regulates oocyte loss via suppressing miR-23b-3p/miR-29a-3p maturation and upregulating STX17 in perinatal mouse ovaries.
Front Vet Sci
Effect of Promoter Methylation on the Expression of Porcine MUC2 Gene and Resistance to PEDV Infection.
Int J Oncol
Upregulation of miR-146a by YY1 depletion correlates with delayed progression of prostate cancer.