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YY1 Polyclonal antibody
YY1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 22156-1-AP
"YY1 Antibodies" Comparison
View side-by-side comparison of YY1 antibodies from other vendors to find the one that best suits your research needs.
|Positive WB detected in||Jurkat cells, A549 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22156-1-AP is YY1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, bovine, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||YY1 fusion protein Ag17792|
|Full Name||YY1 transcription factor|
|Calculated molecular weight||414 aa, 45 kDa|
|Observed molecular weight||65-70 kDa|
|GenBank accession number||BC037308|
|Gene ID (NCBI)||7528|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. This is a rabbit polyclonal antibody raised against full length YY1 of human origin.
|Product Specific Protocols|
|WB protocol for YY1 antibody 22156-1-AP||Download protocol|
|IHC protocol for YY1 antibody 22156-1-AP||Download protocol|
|IF protocol for YY1 antibody 22156-1-AP||Download protocol|
|IP protocol for YY1 antibody 22156-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Sci Transl Med
Yin Yang 1 protein ameliorates diabetic nephropathy pathology through transcriptional repression of TGFβ1.
Dynamic control of chromatin-associated m6A methylation regulates nascent RNA synthesis.
KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia.
MORTALIN-Ca2+ axis drives innate rituximab resistance in diffuse large B-cell lymphoma.
Free Radic Biol Med
Asymmetrical arginine dimethylation of histone H4 by 8-oxog/OGG1/PRMT1 is essential for oxidative stress-induced transcription activation.
HPV8 Reverses the Transcriptional Output in Lrig1 Positive Cells to Drive Skin Tumorigenesis.