|Positive WB detected in||Jurkat cells, A549 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22156-1-AP is YY1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||bovine, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||YY1 fusion protein Ag17792|
|Full Name||YY1 transcription factor|
|Calculated molecular weight||414 aa, 45 kDa|
|Observed molecular weight||65-70 kDa|
|GenBank accession number||BC037308|
|Gene ID (NCBI)||7528|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. This is a rabbit polyclonal antibody raised against full length YY1 of human origin.
|Product Specific Protocols|
|WB protocol for YY1 antibody 22156-1-AP||Download protocol|
|IHC protocol for YY1 antibody 22156-1-AP||Download protocol|
|IF protocol for YY1 antibody 22156-1-AP||Download protocol|
|IP protocol for YY1 antibody 22156-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biochem Biophys Res Commun
YY1-regulated LINC00152 promotes triple negative breast cancer progression by affecting on stability of PTEN protein.
A haplotype variant of Hu sheep follicle-stimulating hormone receptor promoter region decreases transcriptional activity.
Ann Transl Med
Heat shock cognate protein 70 promotes the differentiation of C2C12 myoblast and targets Yin Yang 1.
Int J Oncol
Upregulation of miR-146a by YY1 depletion correlates with delayed progression of prostate cancer.
Changes in MicroRNA Expression During Maturation of the Bovine Corpus Luteum: Regulation of Luteal Cell Proliferation and Function by MicroRNA-34a.
Interleukin-18 Down-Regulates Multidrug Resistance-Associated Protein 2 Expression through Farnesoid X Receptor Associated with Nuclear Factor Kappa B and Yin Yang 1 in Human Hepatoma HepG2 Cells.