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  • KD/KO Validated

YY1 Polyclonal antibody

YY1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IP, IHC, IF, CoIP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 22156-1-AP

Synonyms

DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor


"YY1 Antibodies" Comparison

View side-by-side comparison of YY1 antibodies from other vendors to find the one that best suits your research needs.
Applications:
  • Western Blot (WB) analysis of Jurkat cells using YY1 Polyclonal antibody (22156-1-AP)

    WB analysis of Jurkat using 22156-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using YY1 Polyclonal antibody (22156-1-AP)

    WB analysis of A549 cells using 22156-1-AP

    A549 cells (shcontrol and shRNA of YY1) were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000.

  • Western Blot (WB) analysis of HeLa cells using YY1 Polyclonal antibody (22156-1-AP)

    WB analysis of HeLa using 22156-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using YY1 Polyclonal antibody (22156-1-AP)

    WB analysis of HEK-293 using 22156-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using YY1 Polyclonal antibody (22156-1-AP)

    IP experiment of HeLa using 22156-1-AP

    IP Result of anti-YY1 (IP:22156-1-AP, 5ug; Detection:22156-1-AP 1:700) with HeLa cells lysate 880ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using YY1 Polyclonal antibody (22156-1-AP)

    IHC staining of human breast cancer using 22156-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22156-1-AP (YY1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using YY1 Polyclonal antibody (22156-1-AP)

    IHC staining of human breast cancer using 22156-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22156-1-AP (YY1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using YY1 Polyclonal antibody (22156-1-AP)

    IF Staining of HepG2 using 22156-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 22156-1-AP (YY1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).


Tested Applications

Positive WB detected inJurkat cells, A549 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22156-1-AP is YY1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, bovine, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YY1 fusion protein Ag17792
Full Name YY1 transcription factor
Calculated molecular weight 414 aa, 45 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC037308
Gene symbol YY1
Gene ID (NCBI) 7528
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. This is a rabbit polyclonal antibody raised against full length YY1 of human origin.

Protocols

Product Specific Protocols
WB protocol for YY1 antibody 22156-1-APDownload protocol
IHC protocol for YY1 antibody 22156-1-APDownload protocol
IF protocol for YY1 antibody 22156-1-APDownload protocol
IP protocol for YY1 antibody 22156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Transl Med

Yin Yang 1 protein ameliorates diabetic nephropathy pathology through transcriptional repression of TGFβ1.

Authors - Pan Gao
humanWB

Mol Cell

Dynamic control of chromatin-associated m6A methylation regulates nascent RNA synthesis.

Authors - Wenqi Xu
humanWB

Theranostics

KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia.

Authors - Chengwan Zhang
  • KD Validated
humanChIP

Cancer Lett

MORTALIN-Ca2+ axis drives innate rituximab resistance in diffuse large B-cell lymphoma.

Authors - Qi Sun
humanWB,IP,ChIP

Free Radic Biol Med

Asymmetrical arginine dimethylation of histone H4 by 8-oxog/OGG1/PRMT1 is essential for oxidative stress-induced transcription activation.

Authors - Wentao Wang
mouseIHC

Cancers (Basel)

HPV8 Reverses the Transcriptional Output in Lrig1 Positive Cells to Drive Skin Tumorigenesis.

Authors - Adnan Shahzad Syed