ZAP70 Polyclonal antibody

ZAP70 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 22207-1-AP


70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70

Tested Applications

Positive WB detected inJurkat cells, RAW 264.7 cells, RAW264.7
Positive IP detected inJurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22207-1-AP is ZAP70 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZAP70 fusion protein Ag17527
Full Name zeta-chain (TCR) associated protein kinase 70kDa
Calculated Molecular Weight 619 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC039039
Gene Symbol ZAP70
Gene ID (NCBI) 7535
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.


Product Specific Protocols
WB protocol for ZAP70 antibody 22207-1-APDownload protocol
IHC protocol for ZAP70 antibody 22207-1-APDownload protocol
IF protocol for ZAP70 antibody 22207-1-APDownload protocol
IP protocol for ZAP70 antibody 22207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols