ZBBX Polyclonal antibody

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• ZBBX Polyclonal antibody (23958-1-AP)

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  WB analysis using 23958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23958-1-AP (ZBBX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 23958-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23958-1-AP (ZBBX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ZBBX Polyclonal Antibody for WB, IHC, ELISA

Cat No. 23958-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

Zinc finger B box domain containing protein 1

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Tested Applications

• Positive WB detected in: A549 cells, rat brain tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23958-1-AP targets ZBBX in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20988

  Product name: Recombinant human ZBBX protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-326 aa of BC034229

  Sequence: MEKVQLEFENQEMEKKLQEFRSTRNKEKEDRESSEYYWKSGKVGKLVNQSYMMSQNKGNVVKFSAGKVKLKLLKEQIQEPVKPTVNYKMANSSECEKPKINGKVCGQCENKAALLVCLECGEDYCSGCFANVHQKGALKLHRTTLLQARSQILFNVLDVAHQFIKDVNPDEPKEENNSTKETSKIQHKPKSVLLQRSSSEVEITTMKRAQRTKPRKSLLCEGSFDEEASAQSFQEVLSQWRTGNHDDNKKQNLHAAVKDSLEECEVQTNLKIWREPLNIELKEDILSYMEKLWLKKHRRTPQEQLFKCYQIRSHIHMKPLVMHSVL

• Full Name: zinc finger, B-box domain containing
• Calculated Molecular Weight: 800 aa, 91 kDa
• Observed Molecular Weight: 88-96 kDa
• GenBank Accession Number: BC034229
• Gene Symbol: ZBBX
• Gene ID (NCBI): 79740
• RRID: AB_3742099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: A8MT70
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZBBX (Zinc finger B-box domain-containing protein 1) contains a classic B-box-type zinc-finger (B-box1) motif that adopts a C6H2 zinc-binding fold and is therefore predicted to bind zinc ions and mediate protein-protein or protein-nucleic acid interactions. ZBBX was implicated previously by a de novo deletion in a male ADHD proband, and separately by a CNV gain disrupting ZBBX (PMID: 21832240).