ZBTB1 Polyclonal antibody

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  WB analysis of HeLa using 26287-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26287-1-AP (ZBTB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 26287-1-AP

  IP result of anti-ZBTB1 (IP:26287-1-AP, 4ug; Detection:26287-1-AP 1:300) with HeLa cells lysate 4000ug.

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  IF Staining of HeLa using 26287-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 26287-1-AP (ZBTB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

ZBTB1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 26287-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, ELISA

Synonyms

KIAA0997, ZBTB1

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

26287-1-AP targets ZBTB1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23492

  Product name: Recombinant human ZBTB1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-357 aa of BC050719

  Sequence: MAKPSHSSYVLQQLNNQREWGFLCDCCIAIDDIYFQAHKAVLAACSSYFRMFFMNHQHSTAQLNLSNMKISAECFDLILQFMYLGKIMTAPSSFEQFKVAMNYLQLYNVPDCLEDIQDADCSSSKCSSSASSKQNSKMIFGVRMYEDTVARNGNEANRWCAEPSSTVNTPHNREADEESLQLGNFPEPLFDVCKKSSVSKLSTPKERVSRRFGRSFTCDSCGFGFSCEKLLDEHVLTCTNRHLYQNTRSYHRIVDIRDGKDSNIKAEFGEKDSSKTFSAQTDKYRGDTSQAADDSASTTGSRKSSTVESEIASEEKSRAAERKRIIIKMEPEDTPTDELKDFNIIKVTDKDCNESTD

• Full Name: zinc finger and BTB domain containing 1
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC050719
• Gene Symbol: ZBTB1
• Gene ID (NCBI): 22890
• RRID: AB_2880463
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y2K1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Metab; ZBTB1 Regulates Asparagine Synthesis and Leukemia Cell Response to L-Asparaginase.; Authors - Robert T Williams ; KO Validated
mouse	WB	iScience; RBFOX2 regulated EYA3 isoforms partner with SIX4 or ZBTB1 to control transcription during myogenesis; Authors - Hannah J Wiedner