ZBTB10 Polyclonal antibody

ZBTB10 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 22944-1-AP

Synonyms

RINZF, RINZFC, ZBTB10, Zinc finger protein RIN ZF

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Validation Data Gallery

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  WB analysis of A375 using 22944-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 22944-1-AP (ZBTB10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 22944-1-AP

  IP result of anti-ZBTB10 (IP:22944-1-AP, 3ug; Detection:22944-1-AP 1:1000) with mouse liver tissue lysate 12500ug.

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  IP experiment using 22944-1-AP

  IP result of ZBTB10 antibody (22944-1-AP for IP and Detection) with mouse liver tissue lysate.

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Tested Applications

• Positive WB detected in: A375 cells
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22944-1-AP is ZBTB10 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZBTB10 fusion protein Ag19094
• Full Name: zinc finger and BTB domain containing 10
• Calculated Molecular Weight: 871 aa, 95 kDa
• Observed Molecular Weight: 65 kDa, 130 kDa
• GenBank Accession Number: BC127098
• Gene Symbol: ZBTB10
• Gene ID (NCBI): 65986
• RRID: AB_2879188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger and BTB domain-containing protein 10 (ZBTB10), also known as RINZF, RINZFC, May be involved in transcriptional regulation. Multiple isoforms of ZBTB10 exist due to alternative splicing events.