ZBTB2 Recombinant monoclonal antibody

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• ZBTB2 Recombinant monoclonal antibody (87621-1-RR)

• 

  WB analysis using 87621-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87621-1-RR (ZBTB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity of 87621-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87621-1-RR against Human ZBTB2 were performed. The affinity constant is below 1 pM.

ZBTB2 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87621-1-RR

Clone No.252923G8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

KIAA1483, Zinc finger and BTB domain-containing protein 2, ZNF437

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HEK-293T cells, U-251 cells, U2OS cells, NIH/3T3 cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87621-1-RR targets ZBTB2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag24041

  Product name: Recombinant human ZBTB2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 165-514 aa of BC020172

  Sequence: RISQEQVPEASQLSQLTSNLAQVNRTNMTPSDPLQTSLSPELVSTPVPPPPPGEETNLEASSSDEQPASLTIAHVKPSIMKRNGSFPKYYACHLCGRRFTLRSSLREHLQIHTGVPFTSSQQGESRVPLTLCSNAADLGKDAMEVPEAGMISDSELQHISDSPIIDGQQQSETPPPSDIADIDNLEQADQEREVKRRKYECTICGRKFIQKSHWREHMYIHTGKPFKCSTCDKSFCRANQAARHVCLNQSIDTYTMVDKQTLELCTFEEGSQMDNMLVQTNKPYKCNLCDKTFSTPNEVVKHSCQNQNSDVFALDEGRSILLGSGDSEVTEPDHPVLASIKKEQETVLLD

• Full Name: zinc finger and BTB domain containing 2
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC020172
• Gene Symbol: ZBTB2
• Gene ID (NCBI): 57621
• RRID: AB_3745656
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N680
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZBTB2 (Zinc Finger and BTB Domain-Containing Protein 2) is a transcriptional regulator belonging to the POK (POZ/BTB and Krüppel) family of zinc finger proteins. It contains an N-terminal BTB (Broad-Complex, Tramtrack, and Bric-à-Brac) domain, which mediates protein-protein interactions and transcriptional repression, and a C-terminal set of Krüppel-type zinc fingers that recognize specific DNA sequences. ZBTB2 is involved in critical cellular processes, including cell cycle progression, differentiation, and DNA damage response.