Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27975-1-AP targets ZBTB24 in WB, IP, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24458 Product name: Recombinant human ZBTB24 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 151-333 aa of BC036731 Sequence: SNKKNDPPKRKRGRPKKVNTLQEEKSELAAEEEIQLRVNNSVQNRQNFVVKGDSGVLNEQIAAKEKEESEPTCEPSREEEMPVEKDENYDPKTEDGQASQSRYSKRRIWRSVKLKDYKLVGDQEDHGSAKRICGRRKRPGGPEARCKDCGKVFKYNHFLAIHQRSHTGNDVFKADCSVLQNWE Predict reactive species |
| Full Name | zinc finger and BTB domain containing 24 |
| Calculated Molecular Weight | 697 aa, 78 kDa |
| Observed Molecular Weight | 80-85 kDa |
| GenBank Accession Number | BC036731 |
| Gene Symbol | ZBTB24 |
| Gene ID (NCBI) | 9841 |
| RRID | AB_2881026 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43167 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZBTB24 antibody 27975-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZBTB24 Polyclonal antibody (Cat# 27975-1-AP) has 2 total citations. These appear in American Journal of Human Genetics and Clinical and Experimental Immunology. The associated research fields include human genetics (craniofacial and brain abnormalities linked to MN1 truncation variants) and clinical immunology (immune profiling of a patient with immunodeficiency, centromeric instability, and facial anomalies syndrome type 2 caused by a ZBTB24 mutation). Both citations validated the antibody for WB and IP applications in human samples.
| Species | Application | Title |
|---|---|---|
Am J Hum Genet Gain-of-Function MN1 Truncation Variants Cause a Recognizable Syndrome with Craniofacial and Brain Abnormalities. | ||
Clin Exp Immunol In-depth immune profiling of a patient with immunodeficiency, centromeric instability, and facial anomalies syndrome type 2 caused by a novel mutation in ZBTB24 |
Reviews
What customers say
One customer review (4/5 stars) from Guillaume Velasco describes using this antibody on ZBTB24-mutated lymphoblastoid cell lines to validate its specificity. The antibody was tested in Western Blot, Immunoprecipitation, and ChIP applications, with the reviewer confirming reliable performance for these uses.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Guillaume (Verified Customer) (09-08-2019) | I used Lymphoblastoid cell lines mutated for ZBTB24 to validate the specificity of the antibody
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