ZBTB33 Polyclonal antibody, PBS Only

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• ZBTB33 Polyclonal antibody, PBS Only (55270-1-PBS)

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  WB analysis of A431 using 55270-1-AP (same clone as 55270-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 55270-1-AP (ZBTB33 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55270-1-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 55270-1-AP (same clone as 55270-1-PBS)

  IP result of anti-ZBTB33 (IP:55270-1-AP, 4ug; Detection:55270-1-AP 1:300) with A431 cells lysate 1200ug. This data was developed using the same antibody clone with 55270-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 55270-1-AP (same clone as 55270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 55270-1-AP (ZBTB33 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55270-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 55270-1-AP (same clone as 55270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 55270-1-AP (ZBTB33 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55270-1-PBS in a different storage buffer formulation.

ZBTB33 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 55270-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

ZNF348, ZNF kaiso, Zinc finger and BTB domain-containing protein 33, Transcriptional regulator Kaiso, KAISO

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Product Information

55270-1-PBS targets ZBTB33 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: zinc finger and BTB domain containing 33
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 68-74 kDa
• GenBank Accession Number: NM_006777
• Gene Symbol: ZBTB33
• Gene ID (NCBI): 10009
• RRID: AB_2881295
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86T24
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ZBTB33, also named as Transcriptional regulator Kaiso, is a 672 amino acid protein, which localizes in nucleus and cytoplasm. ZBTB33 as a transcriptional regulator with bimodal DNA-binding specificity binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). ZBTB33 recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters and may contribute to the repression of target genes of the Wnt signaling pathway. The calculated molecular weight of ZBTB33 is 75 kDa, but modified protein is about 100 kDa (PMID: 10207085 and PMID: 16354691)