ZBTB38 Polyclonal antibody

ZBTB38 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 21906-1-AP

Synonyms

CIBZ, ZBTB38

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Validation Data Gallery

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  WB analysis using 21906-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21906-1-AP (ZBTB38 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 21906-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 21906-1-AP (ZBTB38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 21906-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21906-1-AP (ZBTB38 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 21906-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21906-1-AP (ZBTB38 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 21906-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21906-1-AP (ZBTB38 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 21906-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21906-1-AP (ZBTB38 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 21906-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21906-1-AP (ZBTB38 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 21906-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21906-1-AP (ZBTB38 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 21906-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21906-1-AP (ZBTB38 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 21906-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21906-1-AP (ZBTB38 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary tumor using 21906-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 21906-1-AP (ZBTB38 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 21906-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 21906-1-AP (ZBTB38 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: PC-3 cells, DU 145 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human testis tissue, human breast cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

21906-1-AP targets ZBTB38 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZBTB38 fusion protein Ag14963
• Full Name: zinc finger and BTB domain containing 38
• Calculated Molecular Weight: 1195 aa, 134 kDa
• Observed Molecular Weight: 134 kDa
• GenBank Accession Number: BC008901
• Gene Symbol: ZBTB38
• Gene ID (NCBI): 253461
• RRID: AB_2878943
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Transl Med; Cisplatin resistance-related multi-omics differences and the establishment of machine learning models.; Authors - Qihai Sui
human	WB,IHC	Cell Death Dis; ZBTB38 suppresses prostate cancer cell proliferation and migration via directly promoting DKK1 expression.; Authors - Guanxiong Ding
human	IHC	J Gynecol Oncol; Prognostication of early-onset endometrioid endometrial cancer based on genome-wide DNA methylation profiles; Authors - Takuro Hirano