ZBTB48 Polyclonal antibody

ZBTB48 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24665-1-AP

Synonyms

TZAP, Telomere zinc finger-associated protein, Protein HKR3, pp9964, Krueppel-related zinc finger protein 3

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Validation Data Gallery

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  WB analysis of K-562 using 24665-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24665-1-AP (ZBTB48 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24665-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24665-1-AP (ZBTB48 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 24665-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24665-1-AP (ZBTB48 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

24665-1-AP targets ZBTB48 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZBTB48 fusion protein Ag20301
• Full Name: zinc finger and BTB domain containing 48
• Calculated Molecular Weight: 688 aa, 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC013573
• Gene Symbol: ZBTB48
• Gene ID (NCBI): 3104
• RRID: AB_2879664
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZBTB48, also named as TZAP and HKR3, is a 688 amino acid protein, which contains 1 BTB (POZ) domain and 11 C2H2-type zinc fingers and belongs to the krueppel C2H2-type zinc-finger protein family. ZBTB48 is detected in adrenal gland and neuroblastoma. ZBTB48 binds to and regulates the J and/or S elements in MHC II promoter. Zinc finger and BTB domain containing 48 (ZBTB48), also known as telomeric zinc-finger associated protein (TZAP), is a protein that triggers the trimming of telomeres in the absence of shelterin. ZBTB48, is a relatively uncharacterized POK family protein with a POZ-domain and 11 zinc finger domains. ZBTB48, is ubiquitously expressed in human tissues. The ZBTB48 gene is mapped to human chromosome 1p36, which is commonly rearranged (leiomyoma and leukemias) or deleted in various cancers (neuroblastoma, melanoma, Merkel cell carcinomas, pheochromocytoma and breast and colon carcinomas. A correlation between deletions or rearrangements of HKR3 and human cancer suggest a role for ZBTB48 as a potential tumor suppressor.

Publications

Species	Application	Title
human	WB	Commun Biol; Reciprocal impacts of telomerase activity and ADRN/MES differentiation state in neuroblastoma tumor biology.; Authors - Eun Young Yu