ZBTB8A Polyclonal antibody

ZBTB8A Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 24544-1-AP

Synonyms

BOZ F1, BOZF1, ZBTB8, ZBTB8A

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Validation Data Gallery

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  WB analysis of HEK-293 using 24544-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24544-1-AP (ZBTB8A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24544-1-AP

  IP result of anti-ZBTB8A (IP:24544-1-AP, 3ug; Detection:24544-1-AP 1:300) with HEK-293 cells lysate 920ug.

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  IF Staining of HEK-293 using 24544-1-AP

  Immunofluorescent analysis of HEK-293 cells using 24544-1-AP (ZBTB8A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24544-1-AP is ZBTB8A Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZBTB8A fusion protein Ag21588
• Full Name: zinc finger and BTB domain containing 8A
• Calculated Molecular Weight: 441 aa, 50 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC015239
• Gene Symbol: ZBTB8A
• Gene ID (NCBI): 653121
• RRID: AB_2879599
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZBTB8A, also named as Zinc finger and BTB domain-containing protein 8A, is a 441 amino acid protein, which may be involved in transcriptional regulation. ZBTB8A may be a potential carcinogenic factor in gastric carcinoma, and may also be involved in gastric adenocarcinoma cell differentiation, cancer invasion and metastasis. The calcualted molecular weight of ZBTB8A is 50 kDa, but modified ZBTB8A is about 55-60 kDa.