ZC3H11A Polyclonal antibody

• 

  WB analysis of mouse ovary using 26081-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 26081-1-AP (ZC3H11A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 26081-1-AP

  WB result of ZC3H11A antibody (26081-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZC3H11A transfected HEK-293 cells.

• 

  WB analysis of HeLa using 26081-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26081-1-AP (ZC3H11A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 26081-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ZC3H11A antibody (26081-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 26081-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ZC3H11A antibody (26081-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

ZC3H11A Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 26081-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA0663, ZC3HDC11A, Zinc finger CCCH domain-containing protein 11A

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Tested Applications

• Positive WB detected in: mouse ovary tissue, HEK-293 cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

26081-1-AP targets ZC3H11A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23424

  Product name: Recombinant human ZC3H11A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 464-810 aa of BC014268

  Sequence: KSMQEVHIKTLEEIKLEKALRVQQSSESSTSSPSQHEATPGARRLLRITKRTGMKEEKNLQEGNEVDSQSSIRTEAKEASGETTGVDITKIQVKRCETMREKHMQKQQEREKSVLTPLRGDVASCNTQVAEKPVLTAVPGITRHLTKRLPTKSSQKVEVETSGIGDSLLNVKCAAQTLEKRGKAKPKVNVKPSVVKVVSSPKLAPKRKAVEMHAAVIAAVKPLSSSSVLQEPPAKKAAVAVVPLVSEDKSVTVPEAENPRDSLVLPPTQSSSDSSPPEVSGPSSSQMSMKTRRLSSASTGKPPLSVEDDFEKLIWEISGGKLEAEIDLDPGKDEDDLLLELSEMIDS

• Full Name: zinc finger CCCH-type containing 11A
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC014268
• Gene Symbol: ZC3H11A
• Gene ID (NCBI): 9877
• RRID: AB_2880368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75152
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZC3H11A is a RNA-binding protein that interacts with purine-rich sequences and is involved in nuclear mRNA export.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
pig	WB	ACS Infect Dis; Porcine ZC3H11A Is Essential for the Proliferation of Pseudorabies Virus and Porcine Circovirus 2.; Authors - Lin Yang ; KO Validated