ZC3H12D Polyclonal antibody

• Featured Product
• KD/KO Validated

ZC3H12D Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 24991-1-AP

Synonyms

MCPIP4, MCP-induced protein 4, MCP induced protein 4, FLJ46041, EC:3.1.-.-

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Validation Data Gallery

• 

  WB analysis of Raji using 24991-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 24991-1-AP (ZC3H12D Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 24991-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24991-1-AP (ZC3H12D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 24991-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 24991-1-AP (ZC3H12D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 24991-1-AP

  IP result of anti-ZC3H12D (IP:24991-1-AP, 4ug; Detection:24991-1-AP 1:600) with Raji cells lysate 2000ug.

• 

  IHC staining of human tonsillitis using 24991-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24991-1-AP (ZC3H12D Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 24991-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24991-1-AP (ZC3H12D antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 24991-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24991-1-AP (ZC3H12D Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: Raji cells, K-562 cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Product Information

24991-1-AP targets ZC3H12D in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZC3H12D fusion protein Ag21770
• Full Name: zinc finger CCCH-type containing 12D
• Calculated Molecular Weight: 527 aa, 58 kDa
• Observed Molecular Weight: 55-58 kDa
• GenBank Accession Number: BC157832
• Gene Symbol: ZC3H12D
• Gene ID (NCBI): 340152
• RRID: AB_2879834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZC3H12D, also named as MCP induced protein 4, is a 527 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12D exists as three isoforms and localizes in the cytoplasm. ZC3H12D may regulate cell growth likely by suppressing RB1 phosphorylation and serves as a tumor suppressor in certain leukemia cells. ZC3H12D is expressed in normal human lymphocytes but defective in some leukemia/lymphoma cell lines.

Publications

Species	Application	Title
human	IHC	Cell Rep Med; Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application; Authors - Xiaoxue Ren
	WB	Nat Commun; Extracellular mRNA transported to the nucleus exerts translation-independent function.; Authors - Takeshi Tomita ; KO Validated
human	WB	Comput Struct Biotechnol J; Integrating knowledge of protein sequence with protein function for the prediction and validation of new MALT1 substrates; Authors - Peter A Bell
mouse	WB	J Biol Chem; Monocyte Chemotactic Protein-induced Protein 1 and 4 Form a Complex but Act Independently in Regulation of Interleukin-6 mRNA Degradation.; Authors - Shengping Huang ; KD Validated
human	WB	Front Aging Neurosci; DNA Methylation Profiling Reveals the Change of Inflammation-Associated ZC3H12D in Leukoaraiosis.; Authors - Wen-Qing Huang
human	WB	Oncol Lett; miR-128-3p serves as an oncogenic microRNA in osteosarcoma cells by downregulating ZC3H12D.; Authors - Maoshu Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Elaine (Verified Customer) (09-06-2024)

Worked very nicely in western blot. Showed one clear band of the correct size in mouse T cell lysate and appears to not have cross-reactivity with Zc3h12a.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 in 5% BSA+TBS-T
• Cell Tissue Type: murine T cells