Tested Applications
Positive WB detected in | mouse brain tissue |
Positive IP detected in | HEK-293T cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
28190-1-AP targets ZC3H14 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen |
CatNo: Ag28182 Product name: Recombinant human ZC3H14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 120-369 aa of NM_001160103 Sequence: MPSARPEKRDSRVSTSSQESKTTNVRQTYDDGAATRLMSTVKPLREPAPSEDVIDIKPEPDDLIDEDLNFVQENPLSQKKPTVTLTYGSSRPSIEIYRPPASRNADSGVHLNRLQFQQQQNSIHAAKQLDMQSSWVYETGRLCEPEVLNSLEETYSPFFRNNSEKMSMEDENFRKRKLPVVSSVVKVKKFNHDGEEEEEDDDYGSRTGSISSSVSVPAKPERRPSLPPSKQANKNLILKAISEAQESVTKT Predict reactive species |
Full Name | zinc finger CCCH-type containing 14 |
Calculated Molecular Weight | 83 aa |
Observed Molecular Weight | 68 kDa |
GenBank Accession Number | NM_001160103 |
Gene Symbol | ZC3H14 |
Gene ID (NCBI) | 79882 |
RRID | AB_2881085 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q6PJT7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for ZC3H14 antibody 28190-1-AP | Download protocol |
IP protocol for ZC3H14 antibody 28190-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |