ZC3H18 Polyclonal antibody

ZC3H18 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25807-1-AP

Synonyms

NHN1, Nuclear protein NHN1, ZC3H18

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Validation Data Gallery

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  WB analysis of HepG2 using 25807-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25807-1-AP (ZC3H18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 25807-1-AP

  IP result of anti-ZC3H18 (IP:25807-1-AP, 4ug; Detection:25807-1-AP 1:600) with HeLa cells lysate 3200 ug.

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  IHC staining of human tonsillitis using 25807-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25807-1-AP (ZC3H18 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cervical cancer using 25807-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25807-1-AP (ZC3H18 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cervical cancer using 25807-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25807-1-AP (ZC3H18 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human tonsillitis using 25807-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25807-1-AP (ZC3H18 Antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IHC detected in: human tonsillitis tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25807-1-AP is ZC3H18 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZC3H18 fusion protein Ag20481
• Full Name: zinc finger CCCH-type containing 18
• Calculated Molecular Weight: 953 aa, 106 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC050463
• Gene Symbol: ZC3H18
• Gene ID (NCBI): 124245
• RRID: AB_2721255
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZC3H18, also named as NHN1, has two CCCH zinc finger domains. It is not essential in bloodstream forms, but in an in vitro model of differentiation. ZC3H18 recruites the TREX protein for SEP1-mediated mRNA export. The gene ZC3H18 has some isoforms with calculated MW 160 kDa and 84 kDa. With phospho modification, the MW of ZC3H18 is about 150 kDa in WB detection.