Tested Applications
| Positive WB detected in | HUVEC cells, A549 cells, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25624-1-AP targets ZC3H7B in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22555 Product name: Recombinant human ZC3H7B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 169-460 aa of BC152558 Sequence: KAYKRPQELETFSLLSNGTAAGVADQGTSNGLGSIDDIETDCYVDPRGSPALLPSTPTMPLFTHVLDLLAPLDSSRTLPSTDSLDDFSDGDVFGPELDTLLDSLSLVQGGLSGSGVPSELPQLIPVFPGGTPLLPPVVGGSIPVSSPLPPASFGLVMDPSKKLAASVLDALDPPGPTLDPLDLLPYSETRLDALDSFGSTRGSLDKPDSFMEETNSQDHRPTSGAQKPAPSPEPCMPNTALLIKNPLAATHEFKQACQLCYPKTGPRAGDYTYREGLEHKCKRDILLGRLRS Predict reactive species |
| Full Name | zinc finger CCCH-type containing 7B |
| Calculated Molecular Weight | 993 aa, 112 kDa |
| Observed Molecular Weight | 111 kDa |
| GenBank Accession Number | BC152558 |
| Gene Symbol | ZC3H7B |
| Gene ID (NCBI) | 23264 |
| RRID | AB_2880166 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UGR2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZC3H7B antibody 25624-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZC3H7B Polyclonal antibody (Cat# 25624-1-AP) has 3 citations published in Acta Neuropathologica and Cell Reports. The cited studies span neurodegeneration (alpha-synuclein–induced mitochondrial oxidative damage), RNA biology (ZC3H7A/ZC3H7B-mediated mRNA translation and stability), and cancer (mutant p53 RNA-binding activity reprogramming translation and mitochondrial function).
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity. | ||
Cell Rep Coordinated regulation of mRNA translation and stability by ZC3H7A and ZC3H7B RNA-binding proteins. | ||
Cell Rep Mutant p53 gains RNA-binding activity to reprogram translation and mitochondrial function. |
Reviews
What customers say
The single review for this product highlights its strong performance in Western Blot. The reviewer, who had tested multiple antibodies for this protein, found this one to be the best in terms of both specificity and sensitivity of detection. It was used at a 1:1000 dilution and received a 4-star rating, reflecting overall satisfaction with its reliable and clear results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
parisa (Verified Customer) (07-06-2021) | I tried different antibodies for this protein and this product was the best one for specificity and sensitivity of detection in WB.
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