ZC3H7B Polyclonal antibody

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  WB analysis of HUVEC using 25624-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 25624-1-AP (ZC3H7B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25624-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25624-1-AP (ZC3H7B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 25624-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 25624-1-AP (ZC3H7B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25624-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25624-1-AP (ZC3H7B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25624-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25624-1-AP (ZC3H7B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 25624-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 25624-1-AP (ZC3H7B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

ZC3H7B Polyclonal Antibody for WB, ELISA

Cat No. 25624-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

KIAA1031, Rotavirus 'X'-associated non-structural protein, RoXaN, Zinc finger CCCH domain-containing protein 7B

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Tested Applications

• Positive WB detected in: HUVEC cells, A549 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25624-1-AP targets ZC3H7B in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZC3H7B fusion protein Ag22555
• Full Name: zinc finger CCCH-type containing 7B
• Calculated Molecular Weight: 993 aa, 112 kDa
• Observed Molecular Weight: 111 kDa
• GenBank Accession Number: BC152558
• Gene Symbol: ZC3H7B
• Gene ID (NCBI): 23264
• RRID: AB_2880166
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UGR2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		Acta Neuropathol; Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity.; Authors - Di Hu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

parisa (Verified Customer) (07-06-2021)

I tried different antibodies for this protein and this product was the best one for specificity and sensitivity of detection in WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

ZC3H7B Antibody Western Blot validation (1:1000 dilution) in (Cat no:25624-1-AP)