ZC3HAV1L Polyclonal antibody

ZC3HAV1L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20944-1-AP

Synonyms

C7orf39, ZC3HAV1L

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Validation Data Gallery

• 

  WB analysis of Jurkat using 20944-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20944-1-AP (ZC3HAV1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20944-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20944-1-AP (ZC3HAV1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20944-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20944-1-AP (ZC3HAV1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SW 1990 using 20944-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 20944-1-AP (ZC3HAV1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 20944-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20944-1-AP (ZC3HAV1L Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 20944-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20944-1-AP (ZC3HAV1L Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HepG2 cells, SW 1990 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20944-1-AP is ZC3HAV1L Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZC3HAV1L fusion protein Ag15052
• Full Name: zinc finger CCCH-type, antiviral 1-like
• Calculated Molecular Weight: 300 aa, 33 kDa
• Observed Molecular Weight: 33-38 kDa
• GenBank Accession Number: BC008842
• Gene Symbol: ZC3HAV1L
• Gene ID (NCBI): 92092
• RRID: AB_2878771
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.