ZC3HC1 Polyclonal antibody

ZC3HC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13148-1-AP

Synonyms

Zinc finger C3HC-type protein 1, Nuclear-interacting partner of anaplastic lymphoma kinase, Nuclear-interacting partner of ALK, NIPA, HSPC216

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Validation Data Gallery

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  WB analysis using 13148-1-AP

  A431 cell lysates were subjected to SDS PAGE followed by western blot with 13148-1-AP (ZC3HC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 13148-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13148-1-AP (ZC3HC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 13148-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13148-1-AP (ZC3HC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13148-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13148-1-AP (ZC3HC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 13148-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13148-1-AP (ZC3HC1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, mouse skeletal muscle tissue, mouse heart tissue, mouse lung tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13148-1-AP targets ZC3HC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZC3HC1 fusion protein Ag3744
• Full Name: zinc finger, C3HC-type containing 1
• Calculated Molecular Weight: 502 aa, 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC028917
• Gene Symbol: ZC3HC1
• Gene ID (NCBI): 51530
• RRID: AB_2217514
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Proteomics; Analysis of EV71 infection progression using triple-SILAC-based proteomics approach.; Authors - Hao-Yu Li