|Positive WB detected in||PC-3 cells, HeLa cells, MCF-7 cells, mouse brain tissue|
|Positive IP detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Sample-dependent, check data in validation data gallery|
The immunogen of 25196-1-AP is ZCCHC6 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ZCCHC6 fusion protein Ag18240|
|Full Name||zinc finger, CCHC domain containing 6|
|Calculated molecular weight||1495 aa, 171 kDa|
|Observed molecular weight||171 kDa|
|GenBank accession number||BC032456|
|Gene ID (NCBI)||79670|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ZCCHC6 also named as KIAA1711 ,HS2 or TUT7 is a 1495 amino acid protein, which contains threes CCHC-type zinc fingers and two PAP-associated domains. A member of the DNA polymerase type-B-like family, ZCCHC6 as a uridylyltransferase that mediates RNA uridylation.
Critical Roles of Translation Initiation and RNA Uridylation in Endogenous Retroviral Expression and Neural Differentiation in Pluripotent Stem Cells.
The RNA uridyltransferase Zcchc6 is expressed in macrophages and impacts innate immune responses.
TUT7 catalyzes the uridylation of the 3' end for rapid degradation of histone mRNA.
3' Uridylation controls mature microRNA turnover during CD4 T-cell activation.
Identification of RNA-binding proteins that partner with Lin28a to regulate Dnmt3a expression.
RNA uridyl transferases TUT4/7 differentially regulate miRNA variants depending on the cancer cell type.