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  • KD/KO Validated

ZCCHC6 Polyclonal antibody

ZCCHC6 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 25196-1-AP

Synonyms

HS2, KIAA1711, PAPD6, Terminal uridylyltransferase 7, TUT7, TUTase 7, ZCCHC6



Tested Applications

Positive WB detected inPC-3 cells, HeLa cells, MCF-7 cells, mouse brain tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25196-1-AP is ZCCHC6 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZCCHC6 fusion protein Ag18240
Full Name zinc finger, CCHC domain containing 6
Calculated molecular weight 1495 aa, 171 kDa
Observed molecular weight 171 kDa
GenBank accession numberBC032456
Gene symbol ZCCHC6
Gene ID (NCBI) 79670
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ZCCHC6 also named as KIAA1711 ,HS2 or TUT7 is a 1495 amino acid protein, which contains threes CCHC-type zinc fingers and two PAP-associated domains. A member of the DNA polymerase type-B-like family, ZCCHC6 as a uridylyltransferase that mediates RNA uridylation.

Protocols

Product Specific Protocols
WB protocol for ZCCHC6 antibody 25196-1-APDownload protocol
IP protocol for ZCCHC6 antibody 25196-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

Critical Roles of Translation Initiation and RNA Uridylation in Endogenous Retroviral Expression and Neural Differentiation in Pluripotent Stem Cells.

Authors - Kazutoshi Takahashi
mouseWB

PLoS One

The RNA uridyltransferase Zcchc6 is expressed in macrophages and impacts innate immune responses.

Authors - Elyse Kozlowski
  • KO Validated

RNA

TUT7 catalyzes the uridylation of the 3' end for rapid degradation of histone mRNA.

Authors - Patrick E Lackey
humanWB

RNA

3' Uridylation controls mature microRNA turnover during CD4 T-cell activation.

Authors - Cristina Gutiérrez-Vázquez
mouseWB

Sci Rep

Identification of RNA-binding proteins that partner with Lin28a to regulate Dnmt3a expression.

Authors - Silvia Parisi
humanWB

iScience

MiRNA post-transcriptional modification dynamics in T cell activation.

Authors - Ana Rodríguez-Galán