ZCCHC8 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZCCHC8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (2)

Cat no : 23374-1-AP

Synonyms

ZCCHC8

---

Validation Data Gallery

• 

  WB analysis using 23374-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23374-1-AP (ZCCHC8 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 23374-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 23374-1-AP (ZCCHC8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 23374-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 23374-1-AP (ZCCHC8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 23374-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23374-1-AP (ZCCHC8 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 23374-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23374-1-AP (ZCCHC8 antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HepG2 using 23374-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 23374-1-AP (ZCCHC8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, mouse thymus tissue, HepG2 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

23374-1-AP targets ZCCHC8 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZCCHC8 fusion protein Ag19985
• Full Name: zinc finger, CCHC domain containing 8
• Calculated Molecular Weight: 707 aa, 79 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC065918
• Gene Symbol: ZCCHC8
• Gene ID (NCBI): 55596
• RRID: AB_2879269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The eukaryotic RNA exosome participates extensively in RNA processing and degradation. In human cells, three accessory factors (RBM7, ZCCHC8 and hMTR4) interact to form the nuclear exosome targeting (NEXT) complex, which directs a subset of non-coding RNAs for exosomal degradation. ZCCHC8 acts as a scaffold, mediating the interaction between RBM7 and MTR4. NEXT is involved in exosome-mediated surveillance and decay of noncoding RNAs, such as enhancer RNAs (eRNAs) and aberrant 3′-extended transcripts from small nuclear RNA (snRNA), telomerase RNA, and replication-dependent histone genes. NEXT is an important node in regulating nuclear exosome activities and takes part in several biological processes, including DNA damage response, stress response, and viral ribogenesis . ZCCHC8 also has a robust interaction with YTHDC1 (PMID: 28984244).

Publications

Species	Application	Title
mouse	WB,IP	Mol Cell; Nuclear RNA catabolism controls endogenous retroviruses, gene expression asymmetry, and dedifferentiation; Authors - Denis Torre ; KD Validated
human	WB	Nucleic Acids Res; mRNAs are sorted for export or degradation before passing through nuclear speckles.; Authors - Jing Fan
human	CoIP	Nucleic Acids Res; Concurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2.; Authors - Linlin Hou
mouse	WB	Cell Rep; Nuclear Exosome Targeting Complex Core Factor Zcchc8 Regulates the Degradation of LINE1 RNA in Early Embryos and Embryonic Stem Cells.; Authors - You Wu ; KO Validated
human	WB	Nat Neurosci; Globally reduced N6-methyladenosine (m6A) in C9ORF72-ALS/FTD dysregulates RNA metabolism and contributes to neurodegeneration; Authors - Yini Li ; KD Validated
human	WB	PLoS Genet; RBMX involves in telomere stability maintenance by regulating TERRA expression; Authors - Jingfan Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Moritz (Verified Customer) (08-20-2024)

I used this antibody to detect ZCCHC8 in HEK293T WT cell lysate on Western blot, which worked perfectly. The signal was very specific, no unspecific bands were detectable.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T WT

FH

Roy (Verified Customer) (06-15-2024)

Perfect for detecting the NEXT complex component ZCCHC8 by WB (1h incubation at room temperature, 1/1000 dilution)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa