Tested Applications
| Positive WB detected in | mouse kidney tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24759-1-AP targets ZDHHC13 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21607 Product name: Recombinant human ZDHHC13 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 529-622 aa of BC056152 Sequence: FHFSWSTFLLLNQLFQIAFLGLTSHERISLQKQSKHMKQTLSLRKTPYNLGFMQNLADFFQCGCFGLVKPCVVDWTSQYTMVFHPAREKVLRSV Predict reactive species |
| Full Name | zinc finger, DHHC-type containing 13 |
| Calculated Molecular Weight | 622 aa, 71 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC056152 |
| Gene Symbol | ZDHHC13 |
| Gene ID (NCBI) | 54503 |
| RRID | AB_2879708 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IUH4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Palmitoyltransferase ZDHHC13 also named as HIP14L or DHHC13 is a 622 amino acid protein, which contains one DHHC-type zinc finger and six ANK repeats. ZDHHC13 localizes in the Golgi apparatus membrane and belongs to the DHHC palmitoyltransferase family. ZDHHC13 is a palmtoyltransferase for HD and GAD2, meanwhile it mediates Mg2+ transportation. ZDHHC13 exists as three alternative splicing isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZDHHC13 antibody 24759-1-AP | Download protocol |
| WB protocol for ZDHHC13 antibody 24759-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZDHHC13 Polyclonal antibody (Cat# 24759-1-AP) has 2 citations. They appear in Journal of Cell Biology (Golgi turnover and selective autophagy) and Journal of Physiology and Biochemistry (GSDMD palmitoylation and pyroptosis in acute pancreatitis). Research fields span Golgi biology, autophagy, lipid modification, and inflammatory cell death.
| Species | Application | Title |
|---|---|---|
J Physiol Biochem GSDMD palmitoylation induces acinar cell pyroptosis via promoting GSDMD-N oligomerization to aggravate hyperlipidemic acute pancreatitis. |
