HIP14; ZDHHC17 Polyclonal antibody

• Featured Product
• KD/KO Validated

HIP14; ZDHHC17 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15465-1-AP

Synonyms

DHHC 17, HIP 14, HIP 3, HIP14, HIP14; ZDHHC17, HIP3, HSPC294, Huntingtin yeast partner H, HYPH, KIAA0946, Palmitoyltransferase ZDHHC17, ZDHHC17

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Validation Data Gallery

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  WB analysis of Raji using 15465-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15465-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 15465-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15465-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 15465-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15465-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15465-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15465-1-AP (HIP14; ZDHHC17 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Raji cells, human kidney tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

15465-1-AP targets HIP14; ZDHHC17 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HIP14; ZDHHC17 fusion protein Ag7763
• Full Name: zinc finger, DHHC-type containing 17
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC050324
• Gene Symbol: ZDHHC17
• Gene ID (NCBI): 23390
• RRID: AB_2217690
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Post-translational modification by the lipid palmitate is crucial for the correct targeting and function of many proteins. Polyglutamine expansions of huntingtin protein are responsible for the Huntington neurological disorder. ZDHHC17 gene encodes palmitoyl transferase huntingtin interacting protein (HIP14) which regulates palmitoylation and distribution of hungtingtin. The latter is implicated in the formation of inclusion bodies and enhanced neuronal toxicity. HIP14 was also shown to control neurotransmitter release.

Publications

Species	Application	Title
human	IF, WB	J Cell Biol; Regulation of Golgi turnover by CALCOCO1-mediated selective autophagy.; Authors - Thaddaeus Mutugi Nthiga
human	WB	mBio; Identification of ZDHHC17 as a Potential Drug Target for Swine Acute Diarrhea Syndrome Coronavirus Infection; Authors - Yun Luo ; KO Validated
	WB	J Cell Sci; Activity-dependent post-translational regulation of palmitoylating and depalmitoylating enzymes in the hippocampus; Authors - Danya Abazari
mouse	IHC	Mol Cell Endocrinol; ZDHHC17 participates in the pathogenesis of polycystic ovary syndrome by affecting androgen conversion to estrogen in granulosa cells; Authors - Shanmeizi Zhao