ZDHHC5 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84803-4-RR (same clone as 84803-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84803-4-RR (ZDHHC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84803-4-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 84803-4-RR (same clone as 84803-4-PBS)

  IP result of anti-ZDHHC5 (IP:84803-4-RR, 4ug; Detection:84803-4-RR 1:1000) with HEK-293 cells lysate 1720 ug. This data was developed using the same antibody clone with 84803-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84803-4-RR (same clone as 84803-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84803-4-RR (ZDHHC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84803-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84803-4-RR (same clone as 84803-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84803-4-RR (ZDHHC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84803-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84803-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84803-4-RR against Human ZDHHC5 were performed. The affinity constant is 2.16 nM.

ZDHHC5 Uni-rAb^® Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 84803-4-PBS

Clone No.242192A4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

242192A4, DHHC-5, EC:2.3.1.225, KIAA1748, Palmitoyltransferase ZDHHC5

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Product Information

84803-4-PBS targets ZDHHC5 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag15022

  Product name: Recombinant human ZDHHC5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 313-662 aa of BC026967

  Sequence: AMPHSSSAKLSRGDSLKEPTSIAESSRHPSYRSEPSLEPESFRSPTFGKSFHFDPLSSGSRSSSLKSAQGTGFELGQLQSIRSEGTTSTSYKSLANQTRNGSLSYDSLLTPSDSPDFESVQAGPEPDPPLGYTSPFLSARLAQQREAERHPRLVPTGPTHREPSPVRYDNLSRHIVASLQEREKLLRQSPPLPGREEEPGLGDSGIQSTPGSGHAPRTSSSSDDSKRSPLGKTPLGRPAVPRFGKPDGLRGRGVGSPEPGPTAPYLGRSMSYSSQKAQPGVSETEEVALQPLLTPKDEVQLKTTYSKSNGQPKSLGSASPGPGQPPLSSPTRGGVKKVSGVGGTTYEISV

• Full Name: zinc finger, DHHC-type containing 5
• Calculated Molecular Weight: 715 aa, 78 kDa
• Observed Molecular Weight: 75-77 kDa
• GenBank Accession Number: BC026967
• Gene Symbol: ZDHHC5
• Gene ID (NCBI): 25921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9C0B5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The gene encoding zinc finger DHHC-type-containing (ZDHHC)5 is located in a region of chromosome 11q12.1 associated with a high rate of chromosomal translocation and variation, which has been linked to the development of various cancers. ZDHHC5 stimulates the proliferation and anchorage-dependent and -independent colony formation of non-small cell lung cancer cell lines, and was found to be required in a subset of these cells for establishment of tumor xenografts in mice. ZDHHC5 is highly expressed in a variety of embryonic cells including neural progenitor cells, but not in most adult tissues.