Tested Applications
| Positive WB detected in | K-562 cells, HT-1080 cells, NIH/3T3 cells, HSC-T6 cells, 4T1 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67514-1-Ig targets ZEB2 in WB, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25477 Product name: Recombinant human ZEB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 402-658 aa of BC127102 Sequence: VLMATHGFSGTSPFMNGGLGATSPLGVHPSAQSPMQHLGVGMEAPLLGFPTMNSNLSEVQKVLQIVDNTVSRQKMDCKAEEISKLKGYHMKDPCSQPEEQGVTSPNIPPVGLPVVSHNGATKSIIDYTLEKVNEAKACLQSLTTDSRRQISNIKKEKLRTLIDLVTDDKMIENHNISTPFSCQFCKESFPGPIPLHQHERYLCKMNEEIKAVLQPHENIVPNKAGVFVDNKALLLSSVLSEKGMTSPINPYKDHMSV Predict reactive species |
| Full Name | zinc finger E-box binding homeobox 2 |
| Observed Molecular Weight | 160 kDa |
| GenBank Accession Number | BC127102 |
| Gene Symbol | ZEB2 |
| Gene ID (NCBI) | 9839 |
| RRID | AB_2882735 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O60315 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Zinc finger E-box-binding homeobox (ZEB2) is a key transcription factor that acts as a multifunctional regulator during nervous system development. ZEB2 contains two zinc finger domains and a homeodomain-like sequence and interacts with the TGF-ß superfamily signaling regulators, Smads, to regulate the expression of their downstream genes. ZEB2 is expressed in the developing neural tube, as well as in neural crest cells, the hippocampus and the cerebral cortex. ZEB2 has previously been implicated in EMT, cell-cycle progression, apoptosis and senescence. ZEB2 was overexpressed in bladder, ovarian, stomach, pancreatic and squamous cell carcinoma, in the intestinal subtype of stomach cancers, and at the invasive front of CRC where EMT is most prominen. ZEB2 also mediates cell-fate decision in neuronal, T cells and hematopoietic stem cells. The calculated molecular weight of ZEB2 is 136 kDa, but we find the 95 kDa band in some publication(PMID: 27659015 )
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ZEB2 antibody 67514-1-Ig | Download protocol |
| WB protocol for ZEB2 antibody 67514-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZEB2 Monoclonal antibody (Cat# 67514-1-Ig) has 8 citations published in journals including Advances in Science, Journal of Medicinal Chemistry, Frontiers in Immunology, Cancer Gene Therapy, iScience, International Journal of Medical Sciences, Experimental Cell Research, and Experimental Physiology. Research fields span pancreatic, hepatocellular, esophageal, colorectal, and breast cancers, T-cell immunity, and airway remodeling, with a strong focus on epithelial-mesenchymal transition (EMT) signaling pathways.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) ASO Therapy Targeting STAU2 to Inhibit Pancreatic Ductal Adenocarcinoma Progression and Metastasis by Regulating the PALLD-Mediated EMT Signaling Pathway. | ||
J Med Chem Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis. | ||
Front Immunol Endoplasmic reticulum stress-induced CRELD2 promotes APMAP-mediated activation of TGF-β/SMAD and NF-κB pathways in esophageal squamous cell carcinoma | ||
Cancer Gene Ther AMIGO2 enhances the invasive potential of colorectal cancer by inducing EMT | ||
iScience The ADAP-TRIM21-ZEB2 axis regulates CD8(+) T cell effector expansion and central memory-like differentiation. | ||
Int J Med Sci Lnc NR2F1-AS1 Promotes Breast Cancer Metastasis by Targeting the MiR-25-3p/ZEB2 Axis
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