ZER1 Polyclonal antibody

ZER1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16647-1-AP

Synonyms

C9orf60, Hzyg, Protein zer 1 homolog, RP11 545E17.4, zer 1 homolog (C. elegans), ZER1, ZYG, Zyg 11 homolog B like protein, ZYG11BL

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Validation Data Gallery

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  WB analysis using 16647-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16647-1-AP (ZER1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 16647-1-AP

  IP result of anti-ZER1 (IP:16647-1-AP, 3ug; Detection:16647-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of human ovary using 16647-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16647-1-AP (ZER1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 16647-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16647-1-AP (ZER1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 16647-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 16647-1-AP (ZER1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16647-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16647-1-AP (ZER1 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: PC-3 cells, rat testis tissue, mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 16647-1-AP is ZER1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZER1 fusion protein Ag10031
• Full Name: zer-1 homolog (C. elegans)
• Calculated Molecular Weight: 766 aa, 88 kDa
• Observed Molecular Weight: 80-88 kDa
• GenBank Accession Number: BC052563
• Gene Symbol: ZER1
• Gene ID (NCBI): 10444
• RRID: AB_2878294
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.