ZER1 Polyclonal antibody

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  WB analysis using 16647-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16647-1-AP (ZER1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 16647-1-AP

  WB result of ZER1 antibody (16647-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZER1 transfected LNCaP cells.

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  IP experiment of mouse testis using 16647-1-AP

  IP result of anti-ZER1 (IP:16647-1-AP, 4ug; Detection:16647-1-AP 1:1000) with mouse testis tissue lysate 1680 ug.

• Featured Product
• KD/KO Validated

ZER1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 16647-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

C9orf60, Hzyg, Protein zer-1 homolog, ZYG, Zyg-11 homolog B-like protein

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Tested Applications

• Positive WB detected in: LNCaP cells, PC-3 cells, mouse testis tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16647-1-AP targets ZER1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10031

  Product name: Recombinant human ZER1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 324-766 aa of BC052563

  Sequence: RPLQFLGLFENSLCRLTHIPAYKVSGDKNEEQVLNAIEAYTEHRPEITSRAINLLFDIARIERCNQLLRALKLVITALKCHKYDRNIQVTGSAALFYLTNSEYRSEQSVKLRRQVIQVVLNGMESYQEVTVQRNCCLTLCNFSIPEELEFQYRRVNELLLSILNPTRQDESIQRIAVHLCNALVCQVDNDHKEAVGKMGFVVTMLKLIQKKLLDKTCDQVMEFSWSALWNITDETPDNCEMFLNFNGMKLFLDCLKEFPEKQELHRNMLGLLGNVAEVKELRPQLMTSQFISVFSNLLESKADGIEVSYNACGVLSHIMFDGPEAWGVCEPQREEVEERMWAAIQSWDINSRRNINYRSFEPILRLLPQGISPVSQHWATWALYNLVSVYPDKYCPLLIKEGGMPLLRDIIKMATARQETKEMARKVIEHCSNFKEENMDTSR

• Full Name: zer-1 homolog (C. elegans)
• Calculated Molecular Weight: 766 aa, 88 kDa
• Observed Molecular Weight: 80-88 kDa
• GenBank Accession Number: BC052563
• Gene Symbol: ZER1
• Gene ID (NCBI): 10444
• RRID: AB_2878294
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z7L7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.