ZFP161 / ZBTB14 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83909-4-RR (same clone as 83909-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83909-4-RR (ZFP161 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83909-4-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83909-4-RR (same clone as 83909-4-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ZFP161 / ZBTB14 Recombinant antibody (83909-4-RR, Clone:240853B12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83909-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83909-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83909-4-RR against Human ZFP161 / ZBTB14 were performed. The affinity constant is below 1 pM.

ZFP161 / ZBTB14 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83909-4-PBS

Clone No.240853B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

ZFP161, Zfp 5, Zfp 161, ZF5, ZBTB14

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Product Information

83909-4-PBS targets ZFP161 / ZBTB14 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag16125

  Product name: Recombinant human ZFP161 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 103-449 aa of BC110519

  Sequence: EDVNLMMSSGQILGIRFLDKLCSQKRDVSSPDENNGQSKSKYCLKINRPIGDAADTQDDDVEEIGDQDDSPSDDTVEGTPPSQEDGKSPTTTLRVQEAILKELGSEEVRKVNCYGQEVESMETPESKDLGSQTPQALTFNDGMSEVKDEQTPGWTTAASDMKFEYLLYGHHREQIACQACGKTFSDEGRLRKHEKLHTADRPFVCEMCTKGFTTQAHLKEHLKIHTGYKPYSCEVCGKSFIRAPDLKKHERVHSNERPFACHMCDKAFKHKSHLKDHERRHRGEKPFVCGSCTKAFAKASDLKRHENNMHSERKQVTPSAIQSETEQLQAAAMAAEAEQQLETIACS

• Full Name: zinc finger protein 161 homolog (mouse)
• Calculated Molecular Weight: 449 aa, 51 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC110519
• Gene Symbol: ZFP161
• Gene ID (NCBI): 7541
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O43829
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ZBTB14, also named as :ZFP161, ZNF478, is a 449 amino acid protein, which interacts with ZBTB21. ZBTB14, is a transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'. ZBTB14 Binds to 5'-d(GCC)(n)-3' trinucleotide repeats in promoter regions and acts as a repressor of the FMR1 gene.