ZFP36L2 Polyclonal antibody

ZFP36L2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 19005-1-AP

Synonyms

ERF2, ERF 2, EGF-response factor 2, EGF response factor 2, Butyrate response factor 2

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Validation Data Gallery

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  WB analysis of HepG2 using 19005-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19005-1-AP (ZFP36L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 19005-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 19005-1-AP (ZFP36L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 19005-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 19005-1-AP (ZFP36L2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 19005-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19005-1-AP (ZFP36L2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 19005-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19005-1-AP (ZFP36L2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, HL-60 cells
• Positive IHC detected in: human skin cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

19005-1-AP targets ZFP36L2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZFP36L2 fusion protein Ag13518
• Full Name: zinc finger protein 36, C3H type-like 2
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51 kDa, 60 kDa
• GenBank Accession Number: BC005010
• Gene Symbol: ZFP36L2
• Gene ID (NCBI): 678
• RRID: AB_10598773
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Genet; High expression of ZFP36L2 correlates with the prognosis and immune infiltration in lower-grade glioma.; Authors - Min Zhou
zebrafish	WB	J Hum Genet; Genetic association and functional validation of ZFP36L2 in non-syndromic orofacial cleft subtypes; Authors - Jialin Sun
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Zinc finger protein 36 like 2-histone deacetylase 1 axis is involved in the bone responses to mechanical stress; Authors - Bin Wang