ZFYVE16 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZFYVE16 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13118-2-AP

Synonyms

Zinc finger FYVE domain-containing protein 16, KIAA0305, Endosome-associated FYVE domain protein, Endofin

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Validation Data Gallery

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  WB analysis using 13118-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 13118-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13118-2-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13118-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13118-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13118-2-AP

  IP result of anti-ZFYVE16 (IP:13118-2-AP, 5ug; Detection:13118-2-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human kidney using 13118-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 13118-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 13118-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 13118-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 13118-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 13118-2-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 13118-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 13118-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 13118-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZFYVE16 antibody (13118-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). CL594-Phalloidin (red). DAPI (blue)

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, human placenta tissue, mouse kidney tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue, human heart tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

13118-2-AP targets ZFYVE16 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZFYVE16 fusion protein Ag3811
• Full Name: zinc finger, FYVE domain containing 16
• Calculated Molecular Weight: 1539 aa, 169 kDa
• Observed Molecular Weight: 230-250 kDa
• GenBank Accession Number: BC032227
• Gene Symbol: ZFYVE16
• Gene ID (NCBI): 9765
• RRID: AB_2288795
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Endofin (also known as ZFYVE16) is an endosomal FYVE domain protein implicated in regulating membrane trafficking. Endofin has been shown to localize to early endosomes. It co-localizes with SARA but does not associate with it or Smad2 and does not behave like SARA in affecting TGF-signaling. Northern blot analysis showed ZFYVE16 was widely expressed with high levels in kidney, placenta and lung. Overexpression of Endofin causes endosome aggregation. The gene of ZFYVE16 maps to chromosome 5q14, and encodes a 1539-amino acid protein with a molecular mass of 169 kDa. In addition, endogenous Endofin can also be detected as a band of 230-250 kDa. (PMID: 11546807)

Publications

Species	Application	Title
zebrafish	IF	PLoS Genet; The Lowe Syndrome Protein OCRL1 Is Required for Endocytosis in the Zebrafish Pronephric Tubule.; Authors - Francesca Oltrabella
human	WB	Structure; Structural Basis for Specific Interaction of TGFβ Signaling Regulators SARA/Endofin with HD-PTP.; Authors - Deepankar Gahloth ; KD Validated
human	WB	iScience; Endofin is required for HD-PTP and ESCRT-0 interdependent endosomal sorting of ubiquitinated transmembrane cargoes.; Authors - Jalal M Kazan ; KD Validated
human	WB	FEBS Lett; SARA is dispensable for functional TGF-β signaling.; Authors - Bakkeb? Maren M ; KD Validated