ZGPAT Polyclonal antibody

ZGPAT Polyclonal Antibody for WB, ELISA

Cat No. 23203-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

G patch domain-containing protein 6, GPATC6, GPATCH6, KIAA1847, ZC3H9

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis of L02 using 23203-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 23203-1-AP (ZGPAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 23203-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 23203-1-AP (ZGPAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

L02 cells, HepG2 cells,

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23203-1-AP targets ZGPAT in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19620 Product name: Recombinant human ZGPAT protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 173-511 aa of BC032612 Sequence: THKSLKPCPFFLEGKCRFKENCRFSHGQVVSLDELRPFQDPDLSSLQAGSACLAKHQDGLWHAARITDVDNGYYTVKFDSLLLREAVVEGDGILPPLRTEATESDSDSDGTGDSSYARVVGSDAVDSGTCSSAFAGWEVHTRGIGSRLLTKMGYEFGKGLGRHAEGRVEPIHAVVLPRGKSLDQCVETLQKQTRVGKAGTNKPPRCRGRGARPGGRPAPRNVFDFLNEKLQGQAPGALEAGAAPAGRRSKDMYHASKSAKRALSLRLFQTEEKIERTQRDIRSIQEALARNAGRHSVASAQLQEKLAGAQRQLGQLRAQEAGLQQEQRKADTHKKMTEF Predict reactive species
Full Name	zinc finger, CCCH-type with G patch domain
Calculated Molecular Weight	531 aa, 57 kDa
Observed Molecular Weight	65 kDa
GenBank Accession Number	BC032612
Gene Symbol	ZGPAT
Gene ID (NCBI)	84619
RRID	AB_2879231
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	Q8N5A5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZGPAT (Zinc finger CCCH-type with G patch domain-containing protein), also known as zinc finger CCCH domain-containing protein 9 (ZC3HDC9) and G patch domain-containing protein 6 (GPATC6), is a 531 amino acid protein that contains a G-patch domain, which is typically found within RNA-binding proteins. Proteins that contain the G-patch domain include some tumor suppressor and DNA-damage repair proteins. ZGPAT also contains one C3H1-type zinc finger, which further supports its probable role as an RNA-binding protein. The gene encoding ZGPAT is inactivated via differential methylation in a oligodendroglioma cell line, suggesting that ZGPAT may have utility as a biomarker. There are several isoforms of ZGPAT that are produced as a result of alternative splicing events.

Protocols

Product Specific Protocols
WB protocol for ZGPAT antibody 23203-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The ZGPAT Polyclonal antibody (Cat# 23203-1-AP) has 1 citation. It appears in Nature Communications (IF 18.1), in a 2025 study by Mai Morita et al. titled "Combinatorial ubiquitin code degrades deubiquitylation-protected substrates." The research field is ubiquitin-mediated protein degradation, specifically exploring how combinatorial ubiquitin codes target substrates protected from deubiquitylation for proteasomal degradation. The antibody was used in Western blot (WB) with human samples.