ZGPAT Polyclonal antibody

ZGPAT Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 23203-1-AP

Synonyms

ZC3HDC9, ZC3H9, KIAA1847, GPATCH6, GPATC6

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Validation Data Gallery

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  WB analysis of L02 using 23203-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 23203-1-AP (ZGPAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 23203-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23203-1-AP (ZGPAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: L02 cells, HepG2 cells,

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23203-1-AP targets ZGPAT in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZGPAT fusion protein Ag19620
• Full Name: zinc finger, CCCH-type with G patch domain
• Calculated Molecular Weight: 531 aa, 57 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC032612
• Gene Symbol: ZGPAT
• Gene ID (NCBI): 84619
• RRID: AB_2879231
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZGPAT (Zinc finger CCCH-type with G patch domain-containing protein), also known as zinc finger CCCH domain-containing protein 9 (ZC3HDC9) and G patch domain-containing protein 6 (GPATC6), is a 531 amino acid protein that contains a G-patch domain, which is typically found within RNA-binding proteins. Proteins that contain the G-patch domain include some tumor suppressor and DNA-damage repair proteins. ZGPAT also contains one C3H1-type zinc finger, which further supports its probable role as an RNA-binding protein. The gene encoding ZGPAT is inactivated via differential methylation in a oligodendroglioma cell line, suggesting that ZGPAT may have utility as a biomarker. There are several isoforms of ZGPAT that are produced as a result of alternative splicing events.