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  • KD/KO Validated

ZHX2 Polyclonal antibody

ZHX2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20136-1-AP

Synonyms

AFP regulator 1, AFR1, Alpha fetoprotein regulator 1, KIAA0854, RAF, Regulator of AFP, ZHX2, zinc fingers and homeoboxes 2



Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue, mouse skeletal muscle tissue, mouse brain tissue, mouse ovary tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20136-1-AP is ZHX2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZHX2 fusion protein Ag14088
Full Name zinc fingers and homeoboxes 2
Calculated molecular weight 837 aa, 92 kDa
Observed molecular weight 92-100 kDa
GenBank accession numberBC042145
Gene symbol ZHX2
Gene ID (NCBI) 22882
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZHX2 antibody 20136-1-APDownload protocol
IHC protocol for ZHX2 antibody 20136-1-APDownload protocol
IF protocol for ZHX2 antibody 20136-1-APDownload protocol
IP protocol for ZHX2 antibody 20136-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

FEBS Open Bio

Upregulation of ZHX2 predicts poor prognosis and is correlated with immune infiltration in gastric cancer.

Authors - Anqi Cheng
humanWB

Biochem Biophys Res Commun

Proliferation and osteo/odontogenic differentiation of stem cells from apical papilla regulated by Zinc fingers and homeoboxes 2: An in vitro study.

Authors - Fang Wan
  • KD Validated
mouseWB

J Immunol

Zhx2 Accelerates Sepsis by Promoting Macrophage Glycolysis via Pfkfb3.

Authors - Zehua Wang
  • KO Validated
humanWB

J Pathol

ZHX2 inhibits SREBP1c-mediated de novo lipogenesis in hepatocellular carcinoma via miR-24-3p.

Authors - Xiangguo Yu
humanWB

Int J Cancer

HBV suppresses ZHX2 expression to promote proliferation of HCC through miR-155 activation.

Authors - Xiaojia Song