Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, mouse brain tissue, mouse skeletal muscle tissue, mouse ovary tissue, mouse lung tissue, rat brain tissue, Jurkat cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20136-1-AP targets ZHX2 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14088 Product name: Recombinant human ZHX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 488-837 aa of BC042145 Sequence: SDHRYRCQRGIVHITSESLAKDQLAIAASRHGRTYHAYPDFAPQKFKEKTQGQVKILEDSFLKSSFPTQAELDRLRVETKLSRREIDSWFSERRKLRDSMEQAVLDSMGSGKKGQDVGAPNGALSRLDQLSGAQLTSSLPSPSPAIAKSQEQVHLLRSTFARTQWPTPQEYDQLAAKTGLVRTEIVRWFKENRCLLKTGTVKWMEQYQHQPMADDHGYDAVARKATKPMAESPKNGGDVVPQYYKDPKKLCEEDLEKLVTRVKVGSEPAKDCLPAKPSEATSDRSEGSSRDGQGSDENEESSVVDYVEVTVGEEDAISDRSDSWSQAAAEGVSELAESDSDCVPAEAGQA Predict reactive species |
| Full Name | zinc fingers and homeoboxes 2 |
| Calculated Molecular Weight | 837 aa, 92 kDa |
| Observed Molecular Weight | 92-100 kDa |
| GenBank Accession Number | BC042145 |
| Gene Symbol | ZHX2 |
| Gene ID (NCBI) | 22882 |
| RRID | AB_10666438 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6X8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Zinc fingers and homeoboxes protein 2 is a protein that in humans is encoded by the ZHX2 gene. ZHX2 Act as a transcriptional repressor, represses the promoter activity of the CDC25C gene stimulated by NFYA (PMID:12741956). In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZHX2 antibody 20136-1-AP | Download protocol |
| IHC protocol for ZHX2 antibody 20136-1-AP | Download protocol |
| IP protocol for ZHX2 antibody 20136-1-AP | Download protocol |
| WB protocol for ZHX2 antibody 20136-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZHX2 antibody (Cat# 20136-1-AP) has 13 total citations published in journals including Nature Communications, Cancer Letters, Journal of Pathology, International Journal of Cancer, iScience, FASEB Journal, Journal of Immunology, European Journal of Medicinal Chemistry, FEBS Open Bio, and Toxins. Associated research fields encompass liver injury, colorectal and thyroid cancers, hepatocellular carcinoma, bladder cancer, islet β-cell function, vascular remodeling, sepsis, gastric toxicity, stem cell differentiation, and hemangioblastoma.
| Species | Application | Title |
|---|---|---|
Nat Commun ZHX2 emerges as a negative regulator of mitochondrial oxidative phosphorylation during acute liver injury | ||
Cancer Lett Genome-wide CRISPR/Cas9 screen identified MCL1 as a senolytic target for clearing palbociclib-induced senescent and PD-L1-positive cells in colorectal cancer.
| ||
Cancer Cell Int ZHX2 inhibits thyroid cancer metastasis through transcriptional inhibition of S100A14.
| ||
Eur J Med Chem Design, synthesis, and biological evaluation of novel dual inhibitors of heat shock protein 90/mammalian target of rapamycin (Hsp90/mTOR) against bladder cancer cells | ||
J Pathol ZHX2 inhibits SREBP1c-mediated de novo lipogenesis in hepatocellular carcinoma via miR-24-3p. | ||
Int J Cancer HBV suppresses ZHX2 expression to promote proliferation of HCC through miR-155 activation. |
