ZIK1 Polyclonal antibody

ZIK1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 21934-1-AP

Synonyms

ZIK1, Zinc finger protein 762, ZNF762

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Validation Data Gallery

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  WB analysis of Jurkat using 21934-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21934-1-AP (ZIK1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 21934-1-AP

  IP result of anti-ZIK1 (IP:21934-1-AP, 4ug; Detection:21934-1-AP 1:300) with Jurkat cells lysate 2800ug.

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IP detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21934-1-AP is ZIK1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZIK1 fusion protein Ag16568
• Full Name: zinc finger protein interacting with K protein 1 homolog (mouse)
• Calculated Molecular Weight: 487 aa, 55 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC103958
• Gene Symbol: ZIK1
• Gene ID (NCBI): 284307
• RRID: AB_2878948
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.