Phospho-ZIPK (Ser311) Polyclonal antibody

Phospho-ZIPK (Ser311) Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 51021-1-AP

Synonyms

DAP kinase 3, DAP like kinase, DAPK3, Dlk, Phospho-ZIPK (Ser311), ZIP, ZIP kinase, ZIPK

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Validation Data Gallery

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  WB analysis of HEK-293 using 51021-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 51021-1-AP (ZIPK-Phospho 311Ser antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 51021-1-AP

  IP result of anti-Phospho-ZIPK (Ser311) (IP:51021-1-AP, 4ug; Detection:51021-1-AP 1:300) with HEK-293 cells lysate 3200ug.

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  IHC staining of human breast cancer using 51021-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51021-1-AP (Phospho-ZIPK (Ser311) antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

51021-1-AP targets Phospho-ZIPK (Ser311) in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: death-associated protein kinase 3
• Calculated Molecular Weight: 52.5 kDa
• Observed Molecular Weight: 52.5 kDa
• GenBank Accession Number: BC126430
• Gene Symbol: DAPK3
• Gene ID (NCBI): 1613
• RRID: AB_614997
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DAPK3(Death-associated protein kinase 3) is also named ZIPK(Zipper-interacting protein kinase), and Dlk (DAP-like kinase). It is a member of the death-associated protein kinase family associated with apoptosis in nonmuscle cells where it phosphorylates myosin regulatory light chain (RLC) to promote membrane blebbing. DAPK3 mRNA and protein are abundant in heart tissue and isolated ventricular neonatal rat cardiac myocytes(PMID:20038585). It has 2 isoforms produced by alternative splicing, named as ZIPK-L and ZIPK-S. This antibody is specific to ZIPK-Phospho 311Ser.

Publications

Species	Application	Title
human	WB,IF	Cell Signal; Phosphorylation-dependent control of ZIPK nuclear import is species specific.; Authors - Weitzel Douglas H DH