ZMAT3 Polyclonal antibody

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  WB analysis using 10504-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10504-1-AP (ZMAT3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10504-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10504-1-AP (ZMAT3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 10504-1-AP

  IP result of anti-ZMAT3 (IP:10504-1-AP, 3ug; Detection:10504-1-AP 1:700) with mouse testis tissue lysate 4000ug.

ZMAT3 Polyclonal Antibody for WB, IP, ELISA

Cat No. 10504-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

p53 activated gene 608 protein, PAG608, WIG 1, WIG1, Zinc finger protein WIG 1, zinc finger, matrin type 3, ZMAT3

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Tested Applications

• Positive WB detected in: COLO 320 cells, rat testis tissue, HEK-293 cells, HepG2 cells, mouse testis tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

10504-1-AP targets ZMAT3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0780

  Product name: Recombinant human ZMAT3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-289 aa of BC002896

  Sequence: MILLQHAVLPPPKQPSPSPPMSVATRSTGTLQLPPQKPFGQEASLPLAGEEELSKGGEQDCALEELCKPLYCKLCNVTLNSAQQAQAHYQGKNHGKKLRNYYAANSCPPPARMSNVVEPAATPVVPVPPQMGSFKPGGRVILATENDYCKLCDASFSSPAVAQAHYQGKNHAKRLRLAEAQSNSFSESSELGQRRARKEGNEFKMMPNRRNMYTVQNNSAGPYFNPRSRQRIPRDLAMCVTPSGQFYCSMCNVGAGEEMEFRQHLESKQHKSKVSEQRYRNEMENLGYV

• Full Name: zinc finger, matrin type 3
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC002896
• Gene Symbol: ZMAT3
• Gene ID (NCBI): 64393
• RRID: AB_2217579
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HA38
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZMAT3 is a p53-induced zinc finger protein, and a role in the TP53-dependent growth regulatory pathway, contribute to TP53-mediated apoptosis by regulation of TP53 expression and translocation to the nucleus and nucleolus. Acts as a bona fide target gene of p53/TP53.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse		Mol Cell; Zmat3 Is a Key Splicing Regulator in the p53 Tumor Suppression Program.; Authors - Kathryn T Bieging-Rolett
human	WB	J Cell Mol Med; Xanthatin synergizes with cisplatin to suppress homologous recombination through JAK2/STAT4/BARD1 axis in human NSCLC cells.; Authors - Jian Zhang
human	WB,IF,IP	FEBS Lett; The p53 target protein Wig-1 binds hnRNP A2/B1 and RNA Helicase A via RNA.; Authors - Prahl Magdalena M
rat	WB	J Physiol Sci; Tolfenamic acid inhibits ROS-generating oxidase Nox1-regulated p53 activity in intrastriatal injection of malonic acid rats.; Authors - Xin Yang
human	IHC	Int J Gen Med; Upregulation of ZMAT3 is Associated with the Poor Prognosis of Breast Cancer; Authors - Meng Wu
human	WB	Cell Rep; The ribosomal protein L22 binds the MDM4 pre-mRNA and promotes exon skipping to activate p53 upon nucleolar stress; Authors - Jennifer Jansen