ZMAT3 Polyclonal antibody

ZMAT3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 10504-1-AP

Synonyms

p53 activated gene 608 protein, PAG608, WIG 1, WIG1, Zinc finger protein WIG 1, zinc finger, matrin type 3, ZMAT3

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Validation Data Gallery

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  WB analysis using 10504-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10504-1-AP (ZMAT3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10504-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10504-1-AP (ZMAT3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 10504-1-AP

  IP result of anti-ZMAT3 (IP:10504-1-AP, 3ug; Detection:10504-1-AP 1:700) with mouse testis tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: COLO 320 cells, rat testis tissue, HEK-293 cells, HepG2 cells, mouse testis tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

10504-1-AP targets ZMAT3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZMAT3 fusion protein Ag0780
• Full Name: zinc finger, matrin type 3
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC002896
• Gene Symbol: ZMAT3
• Gene ID (NCBI): 64393
• RRID: AB_2217579
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZMAT3 is a p53-induced zinc finger protein, and a role in the TP53-dependent growth regulatory pathway, contribute to TP53-mediated apoptosis by regulation of TP53 expression and translocation to the nucleus and nucleolus. Acts as a bona fide target gene of p53/TP53.

Publications

Species	Application	Title
mouse		Mol Cell; Zmat3 Is a Key Splicing Regulator in the p53 Tumor Suppression Program.; Authors - Kathryn T Bieging-Rolett
human	WB	J Cell Mol Med; Xanthatin synergizes with cisplatin to suppress homologous recombination through JAK2/STAT4/BARD1 axis in human NSCLC cells.; Authors - Jian Zhang
human	WB,IF,IP	FEBS Lett; The p53 target protein Wig-1 binds hnRNP A2/B1 and RNA Helicase A via RNA.; Authors - Prahl Magdalena M
rat	WB	J Physiol Sci; Tolfenamic acid inhibits ROS-generating oxidase Nox1-regulated p53 activity in intrastriatal injection of malonic acid rats.; Authors - Xin Yang
human	IHC	Int J Gen Med; Upregulation of ZMAT3 is Associated with the Poor Prognosis of Breast Cancer; Authors - Meng Wu
human	WB	Cell Rep; The ribosomal protein L22 binds the MDM4 pre-mRNA and promotes exon skipping to activate p53 upon nucleolar stress; Authors - Jennifer Jansen