ZMIZ1-Specific Polyclonal antibody

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  WB analysis using 16159-1-AP

  SK-BR-3 cell lysates were subjected to SDS PAGE followed by western blot with 16159-1-AP (ZMIZ1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 16159-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ZMIZ1-Specific antibody (16159-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

ZMIZ1-Specific Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 16159-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ZMIZ1, RAI17, PIAS-like protein Zimp10, PIAS like protein Zimp10, MIZ

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Tested Applications

• Positive WB detected in: SK-BR-3 cells
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16159-1-AP targets ZMIZ1-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: zinc finger, MIZ-type containing 1
• Calculated Molecular Weight: 115 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: NM_020338
• Gene Symbol: ZMIZ1
• Gene ID (NCBI): 57178
• RRID: AB_3669232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9ULJ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZMIZ2 and ZMIZ1 are members of a PIAS-like family of proteins that interact with nuclear hormone receptors. It can act as a transcriptional coactivator, increasing ligand-dependent transcriptional activity of AR and promoting AR sumoylation. The stimulation of AR activity is dependent upon sumolylation. This antibody is specific for ZMIZ1.