ZMYND10 Polyclonal antibody

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  WB analysis using 14431-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14431-1-AP (ZMYND10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 14431-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14431-1-AP (ZMYND10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 14431-1-AP

  IP result of anti-ZMYND10 (IP:14431-1-AP, 4ug; Detection:14431-1-AP 1:500) with SH-SY5Y cells lysate 1600ug.

• Featured Product
• KD/KO Validated

ZMYND10 Polyclonal Antibody for WB, IP, ELISA

Cat No. 14431-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IP, ELISA

Synonyms

BLU, LUCA12.4, Protein BLu, Zinc finger MYND domain-containing protein 10

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Tested Applications

• Positive WB detected in: human testis tissue, SH-SY5Y cells, mouse testis tissue, rat testis tissue
• Positive IP detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

14431-1-AP targets ZMYND10 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZMYND10 fusion protein Ag5808
• Full Name: zinc finger, MYND-type containing 10
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC033732
• Gene Symbol: ZMYND10
• Gene ID (NCBI): 51364
• RRID: AB_2218002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75800
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZMYND10 (Zinc finger MYND domain-containing protein 10), also known as BLU, encodes a 50-kDa protein containing an MYND-type zinc finger DNA-binding domain in the C-terminus that is commonly found in transcription repressors. ZMYND10 is highly enriched in ciliated cells compared with that in nonciliated cells and is expressed in motile ciliated tissues in mice (PMID: 23891471). ZMYND10 is a tumor suppressor that can induce apoptosis, arrest cell cycle, and inhibit proliferation and angiogenesis in different tumors (PMID: 29601588, 31801619).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IP,IF	Elife; ZMYND10 functions in a chaperone relay during axonemal dynein assembly.; Authors - Girish R Mali ; KO Validated
human	WB,IF	Andrology; A novel homozygous LRRC6 mutation causes male infertility with asthenozoospermia and primary ciliary dyskinesia in humans; Authors - Shengjia Shi
rat	WB	Environ Toxicol; Sodium arsenite impairs sperm quality via downregulating the ZMYND15 and ZMYND10; Authors - Xiangli Li