ZMYND10 Recombinant antibody

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  WB analysis using 86430-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86430-1-RR (ZMYND10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86430-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86430-1-RR (ZMYND10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86430-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86430-1-RR against Human ZMYND10 were performed. The affinity constant is below 1 pM.

ZMYND10 Recombinant Antibody for WB, ELISA

Cat No. 86430-1-RR

Clone No.251276H4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

BLU, LUCA12.4, Protein BLu, Zinc finger MYND domain-containing protein 10

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse kidney tissue, rat kidney tissue, rat testis tissue, human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86430-1-RR targets ZMYND10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ZMYND10 fusion protein Ag5808
• Full Name: zinc finger, MYND-type containing 10
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC033732
• Gene Symbol: ZMYND10
• Gene ID (NCBI): 51364
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75800
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZMYND10 (Zinc finger MYND domain-containing protein 10), also known as BLU, encodes a 50-kDa protein containing an MYND-type zinc finger DNA-binding domain in the C-terminus that is commonly found in transcription repressors. ZMYND10 is highly enriched in ciliated cells compared with that in nonciliated cells and is expressed in motile ciliated tissues in mice (PMID: 23891471). ZMYND10 is a tumor suppressor that can induce apoptosis, arrest cell cycle, and inhibit proliferation and angiogenesis in different tumors (PMID: 29601588, 31801619).