Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25587-1-AP targets ZMYND12 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22358 Product name: Recombinant human ZMYND12 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-365 aa of BC024186 Sequence: MNVIYPLAVPKGRRLCCEVCEAPAERVCAACTVTYYCGVVHQKADWDSIHEKICQLLIPLRTSMPFYNSEEERQHGLQQLQQRQKYLIEFCYTIAQKYLFEGKHEDAVPAALQSLRFRVKLYGLSSVELVPAYLLLAEASLGLGRIVQAEEYLFQAQWTVLKSTDCSNATHSLLHRNLGLLYIAKKNYEEARYHLANDIYFASCAFGTEDIRTSGGYFHLANIFYDLKKLDLADTLYTKVSEIWHAYLNNHYQVLSQAHIQQMDLLGKLFENDTGLDEAQEAEAIRILTSILNIRESTSDKAPQKTIFVLKILVMLYYLMMNSSKAQEYGMRALSLAKEQQLDVHEQSTIQELLSLISTEDHPIT Predict reactive species |
| Full Name | zinc finger, MYND-type containing 12 |
| Calculated Molecular Weight | 365 aa, 42 kDa |
| Observed Molecular Weight | 42-45 kDa |
| GenBank Accession Number | BC024186 |
| Gene Symbol | ZMYND12 |
| Gene ID (NCBI) | 84217 |
| RRID | AB_2880142 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H0C1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZMYND12 antibody 25587-1-AP | Download protocol |
| WB protocol for ZMYND12 antibody 25587-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZMYND12 Polyclonal antibody (Cat# 25587-1-AP) has 1 total citation. It was cited in Cellular and Molecular Life Sciences (2024), in a study investigating ZMYND12's role as an IDAd subunit essential for sperm motility in mice. The research field is reproductive biology and male fertility, with applications validated for WB, IP, and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci ZMYND12 serves as an IDAd subunit that is essential for sperm motility in mice
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