Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, MCF-7 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11633-1-AP targets ZMYND8 in WB, IHC, IF/ICC, ChIP, ELISA, CUT&RUN applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2207 Product name: Recombinant human ZMYND8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 745-1094 aa of BC030721 Sequence: WNSSTVQQKEITQSPSTSTITLVTSTQSSPLVTSSGSMSTLVSSVNADLPIATASADVAADIAKYTSKMMDAIKGTMTEIYNDLSKNTTGSTIAEIRRLRIEIEKLQWLHQQELSEMKHNLELTMAEMRQSLEQERDRLIAEVKKQLELEKQQAVDETKKKQWCANCKKEAIFYCCWNTSYCDYPCQQAHWPEHMKSCTQSATAPQQEADAEVNTETLNKSSQGSSSSTQSAPSETASASKEKETSAEKSKESGSTLDLSGSRETPSSILLGSNQGSVSKRCDKQPAYAPTTTDHQPHPNYPAQKYHSRSNKSSWSSSDEKRGSTRSDHNTSTSTKSLLPKESRLDTFWD Predict reactive species |
| Full Name | zinc finger, MYND-type containing 8 |
| Calculated Molecular Weight | 1186 aa, 132 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC030721 |
| Gene Symbol | ZMYND8 |
| Gene ID (NCBI) | 23613 |
| RRID | AB_2241834 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULU4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Regulation of neuronal gene expression is essential to nervous system development. REST (RE1-silencing transcription factor) regulates neuronal gene expression by interacting with a group of corepressor proteins, including REST corepressors (RCOR). ZMYND8 binds to ROCR2 and can function as a transcriptional repressor. It is co-expressed with RCOR2 in the nervous system, and overexpression of ZMYND8 inhibits neural differentiation. [PMID:20331974]
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZMYND8 antibody 11633-1-AP | Download protocol |
| IHC protocol for ZMYND8 antibody 11633-1-AP | Download protocol |
| WB protocol for ZMYND8 antibody 11633-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZMYND8 Polyclonal antibody (catalog #11633-1-AP) has 12 citations. These appear in high-impact journals including Nature, Molecular Cancer, Nature Cell Biology, Cancer Research, Molecular Cell, Science Advances, Oncogene, Cell Communication and Signaling, Biochemical Pharmacology, and Journal of Tissue Engineering. The research spans cancer biology (ovarian, breast, pancreatic, intestinal), epigenetics and chromatin regulation, transcription, metabolism, and immunology—covering topics such as drug resistance, ZMYND8-mediated ubiquitination, and T cell exhaustion.
| Species | Application | Title |
|---|---|---|
Mol Cancer LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.
| ||
Mol Cancer Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer | ||
Nat Cell Biol Phase separation of ERCC6L2-CtIP regulates the extent of DNA end resection
| ||
Cancer Res KDM5C-mediated recruitment of BRD4 to chromatin regulates enhancer activation and BET inhibitor sensitivity | ||
Mol Cell The ZMYND8-regulated mevalonate pathway endows YAP-high intestinal cancer with metabolic vulnerability.
| ||
