ZMYND8 Recombinant monoclonal antibody

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  WB analysis using 86901-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86901-1-RR (ZMYND8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 86901-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZMYND8 antibody (86901-1-RR, Clone: 251489A6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86901-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86901-1-RR against Human ZMYND8 were performed. The affinity constant is 5.34 nM.

ZMYND8 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86901-1-RR

Clone No.251489A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

CTCL associated antigen se14 3, CTCL-associated antigen se14-3, Cutaneous T-cell lymphoma-associated antigen se14-3, KIAA1125, MYND-type zinc finger-containing chromatin reader ZMYND8

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Tested Applications

• Positive WB detected in: MCF-7 cells, HT-1080 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86901-1-RR targets ZMYND8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2207

  Product name: Recombinant human ZMYND8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 745-1094 aa of BC030721

  Sequence: WNSSTVQQKEITQSPSTSTITLVTSTQSSPLVTSSGSMSTLVSSVNADLPIATASADVAADIAKYTSKMMDAIKGTMTEIYNDLSKNTTGSTIAEIRRLRIEIEKLQWLHQQELSEMKHNLELTMAEMRQSLEQERDRLIAEVKKQLELEKQQAVDETKKKQWCANCKKEAIFYCCWNTSYCDYPCQQAHWPEHMKSCTQSATAPQQEADAEVNTETLNKSSQGSSSSTQSAPSETASASKEKETSAEKSKESGSTLDLSGSRETPSSILLGSNQGSVSKRCDKQPAYAPTTTDHQPHPNYPAQKYHSRSNKSSWSSSDEKRGSTRSDHNTSTSTKSLLPKESRLDTFWD

• Full Name: zinc finger, MYND-type containing 8
• Calculated Molecular Weight: 1186 aa, 132 kDa
• Observed Molecular Weight: 150-170 kDa
• GenBank Accession Number: BC030721
• Gene Symbol: ZMYND8
• Gene ID (NCBI): 23613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9ULU4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Regulation of neuronal gene expression is essential to nervous system development. REST (RE1-silencing transcription factor) regulates neuronal gene expression by interacting with a group of corepressor proteins, including REST corepressors (RCOR). ZMYND8 binds to ROCR2 and can function as a transcriptional repressor. It is co-expressed with RCOR2 in the nervous system, and overexpression of ZMYND8 inhibits neural differentiation. [PMID:20331974]