ZNF124 Polyclonal antibody

ZNF124 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25216-1-AP

Synonyms

HZF 16, HZF16, zinc finger protein 124, Zinc finger protein HZF 16, ZNF124

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 25216-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25216-1-AP (ZNF124 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 25216-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 25216-1-AP (ZNF124 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 25216-1-AP

  IP result of anti-ZNF124 (IP:25216-1-AP, 4ug; Detection:25216-1-AP 1:2000) with SH-SY5Y cells lysate 1600ug.

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  IHC staining of human gliomas using 25216-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25216-1-AP (ZNF124 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 25216-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25216-1-AP (ZNF124 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 25216-1-AP

  Immunofluorescent analysis of HepG2 cells using 25216-1-AP (ZNF124 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, U-937 cells
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25216-1-AP is ZNF124 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF124 fusion protein Ag17165
• Full Name: zinc finger protein 124
• Calculated Molecular Weight: 351 aa, 40 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC099661
• Gene Symbol: ZNF124
• Gene ID (NCBI): 7678
• RRID: AB_2879965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.