ZNF140 Polyclonal antibody

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  WB analysis of SKOV-3 using 24531-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 24531-1-AP (ZNF140 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24531-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24531-1-AP (ZNF140 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 24531-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZNF140 antibody (24531-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

ZNF140 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 24531-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

pHZ 39, Zinc finger protein 140

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Tested Applications

• Positive WB detected in: SKOV-3 cells, Jurkat cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24531-1-AP targets ZNF140 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21379

  Product name: Recombinant human ZNF140 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 79-162 aa of BC040561

  Sequence: SESSGEIKDFSPKNVIYDDSSQYLIMERILSQGPVYSSFKGGWKCKDHTEMLQENQGCIRKVTVSHQEALAQHMNISTVERPYG

• Full Name: zinc finger protein 140
• Calculated Molecular Weight: 457 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC040561
• Gene Symbol: ZNF140
• Gene ID (NCBI): 7699
• RRID: AB_2879592
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P52738
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.