ZNF146 Polyclonal antibody

ZNF146 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20385-1-AP

Synonyms

Only zinc finger protein, OZF, zinc finger protein 146, Zinc finger protein OZF, ZNF146

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Validation Data Gallery

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  WB analysis of mouse kidney using 20385-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20385-1-AP (ZNF146 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 20385-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20385-1-AP (ZNF146 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 20385-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20385-1-AP (ZNF146 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 20385-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20385-1-AP (ZNF146 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse lung tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 20385-1-AP is ZNF146 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF146 fusion protein Ag14223
• Full Name: zinc finger protein 146
• Calculated Molecular Weight: 292 aa, 33 kDa
• Observed Molecular Weight: 20-25 kDa, 33-35 kDa
• GenBank Accession Number: BC005154
• Gene Symbol: ZNF146
• Gene ID (NCBI): 7705
• RRID: AB_10694154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC	J Gastrointest Oncol; Hsa_circ_NOTCH3 regulates ZNF146 through sponge adsorption of miR-875-5p to promote tumorigenesis of hepatocellular carcinoma.; Authors - Lei Bao