ZNF174 Polyclonal antibody

ZNF174 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18157-1-AP

Synonyms

AW 1, zinc finger protein 174, ZNF174, ZSCAN8

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Validation Data Gallery

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  WB analysis of BxPC-3 using 18157-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 18157-1-AP (ZNF174 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 18157-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18157-1-AP (ZNF174 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 18157-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 18157-1-AP

  Immunofluorescent analysis of Hela cells, using ZNF174 antibody 18157-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18157-1-AP targets ZNF174 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZNF174 fusion protein Ag12963
• Full Name: zinc finger protein 174
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC000876
• Gene Symbol: ZNF174
• Gene ID (NCBI): 7727
• RRID: AB_2218459
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.