ZNF18 Polyclonal antibody

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• ZNF18 Polyclonal antibody (17070-1-AP)

ZNF18 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17070-1-AP Copied!

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

HDSG1, Heart development-specific gene 1 protein, KOX11, Zinc finger protein 535, ZKSCAN6

• 

  WB analysis using 17070-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17070-1-AP (ZNF18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 17070-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17070-1-AP (ZNF18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 17070-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17070-1-AP (ZNF18 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 17070-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17070-1-AP (ZNF18 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, HepG2 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17070-1-AP targets ZNF18 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10897

  Product name: Recombinant human ZNF18 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-355 aa of BC036096

  Sequence: MPVDLGQALGLLPSLAKAEDSQFSESDAALQEELSSPETARQLFRQFRYQVMSGPHETLKQLRKLCFQWLQPEVHTKEQILEILMLEQFLTILPGEIQMWVRKQCPGSGEEAVTLVESLKGDPQRLWQWISIQVLGQDILSEKMESPSCQVGEVEPHLEVVPQELGLENSSSGPGELLSHIVKEESDTEAELALAASQPARLEERLIRDRDLGASLLPAAPQEQWRQLDSTQKEQYWDLILETYGKMVSGAGISHPKSDLTNSIEFGEELAGIYLHVNEKIPRPTCIGDRQENDKENLNLENHRDQELLHASCQASGEVPSQASLRGFFTEDEPGCFGEGENLPEALQNIQDEGT

• Full Name: zinc finger protein 18
• Calculated Molecular Weight: 549 aa, 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC036096
• Gene Symbol: ZNF18
• Gene ID (NCBI): 7566
• RRID: AB_2218591
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P17022
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF18 (Zinc Finger Protein 18) is a transcription factor featuring SCAN, KRAB, and C2H2-type zinc finger domains. Predicted to regulate RNA polymerase II-dependent transcription, ZNF18 is ubiquitously expressed, with high levels in the brain and endocrine system. It has been implicated in autism spectrum disorder and heart development.