ZNF189 Monoclonal antibody

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  WB analysis using 68424-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68424-1-Ig (ZNF189 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis of HeLa using 68424-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68424-1-Ig (ZNF189 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ZNF189 Monoclonal Antibody for WB, ELISA

Cat No. 68424-1-Ig

Clone No.2A4B4

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, ELISA

Synonyms

zinc finger protein 189, ZNF189

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, Jurkat cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68424-1-Ig targets ZNF189 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag33164

  Product name: Recombinant human ZNF189 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-150 aa of BC126120

  Sequence: MASPSPPPESKEEWDYLDPAQRSLYKDVMMENYGNLVSLDVLNRDKDEEPTVKQEIEEIEEEVEPQGVIVTRIKSEIDQDPMGRETFELVGRLDKQRGIFLWEIPRESLTQEQRMFRENTNIIRKRPNSEEKCHKCEECGKGFVRKAHFI

• Full Name: zinc finger protein 189
• Observed Molecular Weight: 70-73 kDa
• GenBank Accession Number: BC126120
• Gene Symbol: ZNF189
• Gene ID (NCBI): 7743
• RRID: AB_3085139
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75820
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.