ZNF215 Polyclonal antibody

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  WB analysis of mouse brain using 25502-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25502-1-AP (ZNF215 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 25502-1-AP

  Immunofluorescent analysis of HepG2 cells using 25502-1-AP (ZNF215 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ZNF215 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 25502-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

BAZ2, BWSCR2-associated zinc finger protein 2, Zinc finger protein 215, ZKSCAN11

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25502-1-AP targets ZNF215 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22026

  Product name: Recombinant human ZNF215 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 110-304 aa of BC014538

  Sequence: LQHPNNSKDMVTLIEDVIEMLEDEDMPCKDSALQMGSIKEKMKAGSRTGKPQEPVTFKDVVVEFSKEEWGQLDSAVKNLYRNVMLENFRNLNSLRKAHLLSKPFESLKLESKKKRWIMEKEIPRKTIFDMKSISGEESSHGVIMTRLTESGHPSSDAWKDGVSLLLPRLEYNDAVSAHCNLHLWVQVILLPQPPE

• Full Name: zinc finger protein 215
• Calculated Molecular Weight: 517 aa, 60 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC014538
• Gene Symbol: ZNF215
• Gene ID (NCBI): 7762
• RRID: AB_2880105
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UL58
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF215 (Zinc Finger Protein 215) is a protein-coding gene that acts as a transcription factor, playing a significant role in embryonic development and various genetic disorders. ZNF215 contains SCAN, KRAB, and C2H2-type zinc finger domains. It functions in the nucleus by binding to specific DNA sequences to regulate the expression of downstream genes.